Arrhythpy: an automated tool to quantify and classify arrhythmias in Ca2+ transients of iPSC-cardiomyocytes
Arrhythmias constitute an intricate and clinically significant phenomenon of great importance in various research areas. Calcium (Ca2+) homeostasis plays a pivotal role in forming rhythmic contractions in the heart, and its dysregulation has emerged as a critical component in the development of arrh...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 2025
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| In: |
American journal of physiology. Heart and circulatory physiology
Year: 2025, Volume: 329, Issue: 5, Pages: H1110-H1125 |
| ISSN: | 1522-1539 |
| DOI: | 10.1152/ajpheart.00414.2025 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1152/ajpheart.00414.2025
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| Author Notes: | Karim Ajmail, Charlotte Brand, Thomas Borchert, Benjamin Meder, Sabine Rebs, and Katrin Streckfuss-Bomeke |
MARC
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| 245 | 1 | 0 | |a Arrhythpy |b an automated tool to quantify and classify arrhythmias in Ca2+ transients of iPSC-cardiomyocytes |c Karim Ajmail, Charlotte Brand, Thomas Borchert, Benjamin Meder, Sabine Rebs, and Katrin Streckfuss-Bomeke |
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| 520 | |a Arrhythmias constitute an intricate and clinically significant phenomenon of great importance in various research areas. Calcium (Ca2+) homeostasis plays a pivotal role in forming rhythmic contractions in the heart, and its dysregulation has emerged as a critical component in the development of arrhythmias. However, the quantification of arrhythmias has been limited to indirect measurements via Ca2+ sparks, electrophysiological parameters, or manual classification, which can lead to human bias. We aimed to develop an analysis platform that automatically analyzes arrhythmias in human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Here, we present Arrhythpy, a robust and automated open-source program that quantifies and classifies confocal microscopy-based Fluo-4 Ca2+ transients to generate a measure of arrhythmia. In contrast to other automated and semiautomated analysis tools, which measure established parameters such as time-to-peak, Arrhythpy directly analyzes the degree of arrhythmia in a Ca2+ transient. We demonstrate its utility in monitoring Ca2+ transient-based arrhythmias in atrial and ventricular iPSC-CMs from healthy individuals and patients with cardiac disease, including dilated cardiomyopathy (DCM) and Takotsubo syndrome (TTS). Arrhythpy analysis of iPSC-CMs of patients with TTS recapitulated TTS phenotypes, including atrial arrhythmia that could be normalized with beta-blocker treatment. The program's adaptable framework enables the analysis of arrhythmic patterns in various cell types using periodic dye-based line scan measurement techniques, applicable to both single cells and layered cultures. | ||
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| 650 | 4 | |a ATRIAL | |
| 650 | 4 | |a automated analysis | |
| 650 | 4 | |a CALCIUM | |
| 650 | 4 | |a calcium signaling | |
| 650 | 4 | |a CELL-DERIVED CARDIOMYOCYTES | |
| 650 | 4 | |a human-induced pluripotent stem cell cardiomyocytes | |
| 700 | 1 | |a Brand, Charlotte |e VerfasserIn |0 (DE-588)139148679X |0 (DE-627)1963127560 |4 aut | |
| 700 | 1 | |a Borchert, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Meder, Benjamin |e VerfasserIn |0 (DE-588)135821630 |0 (DE-627)571676316 |0 (DE-576)300664745 |4 aut | |
| 700 | 1 | |a Rebs, Sabine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Streckfuss-Bomeke, Katrin |e VerfasserIn |4 aut | |
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