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A step-by-step guide to performing cancer metabolism research using custom-made media

The preparation of custom-made media offers precise control over nutrient composition, enabling detailed studies of cellular metabolism. We demonstrate how self-made media formulations enable diverse assay designs and readouts to assess cancer metabolism. Self-made media can be used in Seahorse assa...

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Main Authors: Seifert, Sophie (Author) , Yanqui-Rivera, Francisco (Author) , Kühn, Tim (Author) , Høyland, Lena Elise (Author) , Borteçen, Toman (Author) , Agranovich, Bella (Author) , Eckhardt, Lara Elea (Author) , Herdt, Martin (Author) , Henneberg, Alessa L. (Author) , Panitz, Verena (Author) , Hayat, Faisal (Author) , Migaud, Marie (Author) , Poschet, Gernot (Author) , Abramovich, Ifat (Author) , Gottlieb, Eyal (Author) , Krijgsveld, Jeroen (Author) , Ziegler, Mathias (Author) , Prentzell, Mirja Tamara (Author) , Opitz, Christiane (Author)
Format: Article (Journal)
Language:English
Published: February 2026
In: Life science alliance
Year: 2026, Volume: 9, Issue: 2, Pages: 1-26
ISSN:2575-1077
DOI:10.26508/lsa.202503529
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.26508/lsa.202503529
Verlag, kostenfrei, Volltext: https://www.life-science-alliance.org/content/9/2/e202503529
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Author Notes:Sophie Seifert, Francisco Yanqui-Rivera, Tim Kühn, Lena Elise Høyland, Toman Borteçen, Bella Agranovich, Lara Elea Eckhardt, Martin Herdt, Alessa Henneberg, Verena Panitz, Faisal Hayat, Marie Migaud, Gernot Poschet, Ifat Abramovich, Eyal Gottlieb, Jeroen Krijgsveld, Mathias Ziegler, Mirja Tamara Prentzell, Christiane A. Opitz
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Summary:The preparation of custom-made media offers precise control over nutrient composition, enabling detailed studies of cellular metabolism. We demonstrate how self-made media formulations enable diverse assay designs and readouts to assess cancer metabolism. Self-made media can be used in Seahorse assays to measure mitochondrial respiration under defined conditions. In nutrient deprivation experiments, amino acid or vitamin removal can uncover how cancer cells adapt to metabolic stress. Using labeled amino acids enables analysis of nascent protein synthesis and translational regulation, while stable-isotope tracing reveals metabolic fluxes through key pathways. This guide presents a suite of metabolic assays using custom-made media, covering experimental design, the selection of controls, sample preparation, data acquisition, and interpretation. The accompanying online media calculator “Media Minds” streamlines the creation of custom media formulations, ensuring accuracy and reproducibility.
Item Description:Online veröffentlicht: 10. Dezember 2025
Gesehen am 02.03.2026
Physical Description:Online Resource
ISSN:2575-1077
DOI:10.26508/lsa.202503529

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