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Small-RNA sequencing identifies serum microRNAs associated with abnormal electrocardiography findings in patients with Chagas disease

Background - Chagas disease, caused by the parasite Trypanosoma cruzi (T. cruzi), affects around 6-7 million people in Latin America and hundreds of thousands in the United States and Europe. A main complication of chronic Chagas disease is cardiomyopathy, possibly manifesting as arrhythmias, heart...

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Main Authors: Müller, Michael (Author) , Blandino, Alice (Author) , Scherer, Dominique (Author) , Zulantay, Inés (Author) , Apt, Werner (Author) , Varela, Nelson M. (Author) , Llancaqueo, Marcelo (Author) , Garcia, Lineth (Author) , Ortiz, Lourdes (Author) , Nicastri, Emanuele (Author) , Giancola, Maria Letizia (Author) , Angheben, Andrea (Author) , Gabrielli, Simona (Author) , Rounge, Trine B. (Author) , Langseth, Hilde (Author) , Waldenberger, Melanie (Author) , Salinas-Alvarez, Pamela (Author) , Lorenzo Bermejo, Justo (Author)
Format: Article (Journal)
Language:English
Published: 12 September 2025
In: Journal of infection
Year: 2025, Volume: 91, Issue: 4, Pages: 1-9
ISSN:1532-2742
DOI:10.1016/j.jinf.2025.106613
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.jinf.2025.106613
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0163445325002130
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Author Notes:Michael Mueller, Alice Blandino, Dominique Scherer, Inés Zulantay, Werner Apt, Nelson M. Varela, Marcelo Llancaqueo, Lineth Garcia, Lourdes Ortiz, Emanuele Nicastri, Maria Letizia Giancola, Andrea Angheben, Simona Gabrielli, Trine B. Rounge, Hilde Langseth, Melanie Waldenberger, Pamela Salinas-Alvarez, Justo Lorenzo Bermejo
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Summary:Background - Chagas disease, caused by the parasite Trypanosoma cruzi (T. cruzi), affects around 6-7 million people in Latin America and hundreds of thousands in the United States and Europe. A main complication of chronic Chagas disease is cardiomyopathy, possibly manifesting as arrhythmias, heart failure, or sudden cardiac death. Understanding the link between T. cruzi infection and cardiomyopathy is essential for early diagnosis and adequate treatment. - Methods - We sequenced small RNAs in serum samples from 228 Chagas patients recruited in Chile, Bolivia and Italy. After bioinformatic processing of sequencing data to quantify serum miRNA expression, robust logistic regression was applied to identify miRNAs differentially expressed in Chagas patients with abnormalities in electrocardiography (ECG), bundle-branch block on ECG, and high Kuschnir scores. We also investigated the association between genotype-based miRNA expression and the risk of abnormal ECG findings. - Findings - As reported, the risk of abnormal ECG findings was higher in male patients and increased with age. Three miRNAs showed lower serum expression levels in patients with abnormal ECG: miRNA-101-3p, miRNA-576-3p and miRNA-629-5p (p < 0.0002), especially in patients with high Kuschnir scores. The expression of miRNA-629-5p was negatively correlated with the CCL5 expression (p = 3.7×10-8), a chemokine frequently reported in Chagas disease. Gene enrichment analyses indicated involvement of cytokine signalling in Chagas cardiomyopathy. - Interpretation - The findings demonstrate the potential of circulating miRNAs as diagnostic biomarkers for Chagas cardiomyopathy. The associations found with disease severity and immune response may help to improve our knowledge of complications’ development in Chagas disease.
Item Description:Gesehen am 09.04.2026
Physical Description:Online Resource
ISSN:1532-2742
DOI:10.1016/j.jinf.2025.106613

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