PLETHORA-autophagy axis activates organ regeneration through ROS modulation
Injury-induced disruption of cellular homeostasis leads to accumulation of stress at sites adjacent to a wound. How do these cells mitigate wound-induced stress and restore cellular homeostasis to promote regeneration? To address this question, we examined the role of autophagy - a conserved quality...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 10, 2026
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| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 2026, Volume: 123, Issue: 6, Pages: 1-12 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.2513954123 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.2513954123 Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/doi/10.1073/pnas.2513954123 
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| Author Notes: | Akansha Ganguly, Aabha Humnabadkar, Komal Gautam, Viola Willemsen, Lin Xu, Yasin Dagdas, and Kalika Prasad |
| Summary: | Injury-induced disruption of cellular homeostasis leads to accumulation of stress at sites adjacent to a wound. How do these cells mitigate wound-induced stress and restore cellular homeostasis to promote regeneration? To address this question, we examined the role of autophagy - a conserved quality control pathway that recycles defective cellular components to sustain cellular homeostasis - in facilitating wound repair in plants. We demonstrate that transcriptional activation of autophagy-related gene 8 (ATG8) genes is essential for de novo root regeneration, but dispensable for wound induced callus formation from an excised leaf. Plant-specific transcription factors PLETHORA (PLT) activate the transcription of a subset of ATG8 genes and function nonredundantly in this process. Disrupting the PLT-ATG8 regulatory axis severely impairs organelle turnover, resulting in increased intracellular stress and ectopic accumulation of reactive oxygen species (ROS). PLT-ATG8 mediated positioning of optimal ROS levels promotes the expression of stem-cell regulators for successful de novo root regeneration. Altogether, our findings illustrate how plants utilize kingdom-specific developmental regulators, such as PLTs, to activate evolutionarily conserved pathways, effectively managing wound-induced cellular stress and facilitating organ regeneration. |
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| Item Description: | Online veröffentlicht: 30. Januar 2026 Gesehen am 30.04.2026 |
| Physical Description: | Online Resource |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.2513954123 |