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The ribosome-associated N-terminal acetyltransferase B coordinates global proteostasis and autophagy in plants by creating Ac/N-degrons

The N-terminal acetyltransferase B (NatB) acetylates ~20% of the eukaryotic proteome. However, the role of NatB-mediated N-terminal acetylation (NTA) for the regulation of the proteome fate remains unclear in eukaryotes. In this study, we demonstrate that CRISPR-Cas9-mediated deletion of NatB activi...

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Hauptverfasser: Gong, Xiaodi (Verfasst von) , Pożoga, Marlena (Verfasst von) , Boyer, Jean-Baptiste (Verfasst von) , Xue, Yuxing (Verfasst von) , Meinnel, Thierry (Verfasst von) , Bange, Tanja (Verfasst von) , Giglione, Carmela (Verfasst von) , Hell, Rüdiger (Verfasst von) , Wirtz, Markus (Verfasst von)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 31 March 2026
In: Nature Communications
Year: 2026, Jahrgang: 17, Pages: 1-19
ISSN:2041-1723
DOI:10.1038/s41467-026-71208-2
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-026-71208-2
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-026-71208-2
Volltext
Verfasserangaben:Xiaodi Gong, Marlena Pożoga, Jean-Baptiste Boyer, Yuxing Xue, Thierry Meinnel, Tanja Bange, Carmela Giglione, Rüdiger Hell, Markus Wirtz
Beschreibung
Zusammenfassung:The N-terminal acetyltransferase B (NatB) acetylates ~20% of the eukaryotic proteome. However, the role of NatB-mediated N-terminal acetylation (NTA) for the regulation of the proteome fate remains unclear in eukaryotes. In this study, we demonstrate that CRISPR-Cas9-mediated deletion of NatB activity in plants results in significantly lowered global protein turnover due to decreased ubiquitin-proteasome system (UPS) activity and protein translation. Quantitative proteomics uncovers that NatB substrates are significantly enriched in the fraction of stabilized proteins in natb mutants. We provide direct evidence that the absent NTA of KIN11, a subunit of the autophagy-controlling energy sensor SnRK1, protects it from UPS-mediated destruction. The resulting accumulation of KIN11 is responsible for the increased resistance of natb mutants to energy limitation induced by prolonged darkness. Our findings establish NatB as a central regulator of UPS-autophagy interplay and highlight its role in maintaining proteome stability and enabling dynamic stress responses in plants.
Beschreibung:Online verfügbar 31. März 2026
Gesehen am 26.05.2026
Beschreibung:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-026-71208-2

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