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MorphoMapping identifies redox-dependent control of NETotic states in primed neutrophils

Neutrophils rapidly deploy phagocytosis, degranulation, and neutrophil extracellular trap (NET) formation to control infections, yet exaggerated NET formation contributes to tissue injury in inflammatory disease. Because NETosis is tightly linked to the cellular redox environment, we developed Morph...

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Bibliographic Details
Main Authors: Bauerdick, Amelie (Author) , Exner, Tarik (Author) , Jagoditsch, Gabriel (Author) , Habicht, Jüri (Author) , Nakchbandi, Inaam (Author) , Konstandin, Mathias (Author) , Grieshaber-Bouyer, Ricardo (Author) , Wabnitz, Guido H. (Author)
Format: Article (Journal)
Language:English
Published: April 2026
In: The journal of immunology
Year: 2026, Volume: 215, Issue: 4, Pages: 1-20
ISSN:1550-6606
DOI:10.1093/jimmun/vkag061
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/jimmun/vkag061
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Author Notes:Amelie Bauerdick, Tarik Exner, Gabriel Jagoditsch, Jüri Habicht, Inaam Nakchbandi, Mathias Konstandin, Ricardo Grieshaber-Bouyer, and Guido Wabnitz
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Summary:Neutrophils rapidly deploy phagocytosis, degranulation, and neutrophil extracellular trap (NET) formation to control infections, yet exaggerated NET formation contributes to tissue injury in inflammatory disease. Because NETosis is tightly linked to the cellular redox environment, we developed MorphoMapping, an imaging flow cytometry-based pipeline that resolves neutrophil morphotypes at single-cell resolution using purely morphology-derived features. Applying MorphoMapping to cytokine-primed primary human neutrophils exposed to the heme-targeted redox modulator WF10 (tetrachlorodecaoxide) revealed a compact, treatment-specific morphological state and a reproducible redistribution of cells away from NETotic, H3Cit-associated morphotypes. Instead, WF10 enriched nuclear morphologies compatible with non-NETotic termination programs. These data indicate that defined heme-dependent redox modulation suppresses NETotic neutrophil states under priming conditions and establish MorphoMapping as a scalable approach to quantify redox-sensitive neutrophil state shifts in suspension.
Item Description:Gesehen am 19.06.2026
Physical Description:Online Resource
ISSN:1550-6606
DOI:10.1093/jimmun/vkag061

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