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Isolation of a highly virulent colibactin-positive tumor-promoting strain of Escherichia coli from the gut microbiota of an adult

Recent studies have pointed to critical roles for microbes in both exacerbation of and protection from the development of colon cancer. While much has been learned, the field remains understudied, with functional studies available for only a handful of bacteria. To identify novel microbes associated...

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Main Authors: Weis, Allison (Author) , Matthews, O'Connor J. (Author) , Bell, Rickesha (Author) , Pershing, Nicole Lynn (Author) , Dankwardt, Annika (Author) , Fleming, Brittany A. (Author) , Gigić, Biljana (Author) , Schneider, Martin (Author) , Hardikar, Sheetal (Author) , Toriola, Adetunji T. (Author) , Shibata, David (Author) , Li, Christopher I. (Author) , Byrd, Doratha A. (Author) , Stephens, W. Zac (Author) , Ulrich, Cornelia M. (Author) , Mulvey, Matthew A. (Author) , Round, June L. (Author)
Format: Article (Journal)
Language:English
Published: May 26, 2026
In: mSphere
Year: 2026, Volume: 11, Issue: 5, Pages: 1-15
ISSN:2379-5042
DOI:10.1128/msphere.00219-26
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1128/msphere.00219-26
Verlag, kostenfrei, Volltext: https://journals.asm.org/doi/10.1128/msphere.00219-26
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Author Notes:Allison M. Weis, O'Connor J. Matthews, Rickesha Bell, Nicole Lynn Pershing, Annika Dankwardt, Brittany A. Fleming, Biljana Gigic, Martin Schneider, Sheetal Hardikar, Adetunji T. Toriola, David Shibata, Christopher I. Li, Doratha A. Byrd, W. Zac Stephens, Cornelia M. Ulrich, Matthew A. Mulvey, June L. Round
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Summary:Recent studies have pointed to critical roles for microbes in both exacerbation of and protection from the development of colon cancer. While much has been learned, the field remains understudied, with functional studies available for only a handful of bacteria. To identify novel microbes associated with colorectal cancer (CRC) development, we employed a preclinical chemical carcinogenesis CRC mouse model using germ-free mice that were colonized with human microbiotas. During the course of these studies, we identified a microbiota that exacerbated CRC, from which we isolated an Escherichia coli strain that had disseminated to the mouse kidneys. This strain, which we designated as AW001, was genetically similar to the reference adherent-invasive E. coli (AIEC) strain NC101 and encoded the DNA-damaging toxin colibactin. In relevant animal models, AW001 worsened both colitis and sepsis, making it a colitogenic AIEC-like strain with the capacity to cause invasive systemic infections similar to extraintestinal pathogenic E. coli (ExPEC). This strain will be a relev
Item Description:Online veröffentlicht: 07. Mai 2026
Gesehen am 14.08.2026
Physical Description:Online Resource
ISSN:2379-5042
DOI:10.1128/msphere.00219-26

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