Copertina

In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart

The adult zebrafish heart can regenerate after injury, but the earliest gene expression changes that trigger this process remain poorly understood. Here we show that in vivo single-cell RNA metabolic labeling, which marks newly made RNA in individual cells, can capture rapid responses in the adult z...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Mintcheva, Janita (Autore) , Tseng, Tzu-Lun (Autore) , Goumenaki, Pinelopi (Autore) , Neuschulz, Anika (Autore) , Senoussi, Anis (Autore) , Lelek, Sara (Autore) , Lim, Khai Lone (Autore) , Ming, Zhitao (Autore) , Schäfer, Ronny (Autore) , Hnatiuk, Alisa (Autore) , Ninov, Nikolay (Autore) , Beisaw, Arica (Autore) , McGrail, Maura (Autore) , Lai, Shih-Lei Ben (Autore) , Panáková, Daniela (Autore) , Stainier, Didier Y. R. (Autore) , Junker, Jan Philipp (Autore)
Natura: Article (Journal)
Lingua:inglese
Pubblicazione: 05 May 2026
In: Nature Communications
Year: 2026, Volume: 17, Pages: 1-16
ISSN:2041-1723
DOI:10.1038/s41467-026-72781-2
Accesso online:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-026-72781-2
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-026-72781-2
Testo
Note sull'autore:Janita Mintcheva, Tzu-Lun Tseng, Pinelopi Goumenaki, Anika Neuschulz, Anis Senoussi, Sara Lelek, Khai Lone Lim, Zhitao Ming, Ronny Schäfer, Alisa Hnatiuk, Nikolay Ninov, Arica Beisaw, Maura McGrail, Shih-Lei Ben Lai, Daniela Panáková, Didier Y.R. Stainier & Jan Philipp Junker
Descrizione
Riassunto:The adult zebrafish heart can regenerate after injury, but the earliest gene expression changes that trigger this process remain poorly understood. Here we show that in vivo single-cell RNA metabolic labeling, which marks newly made RNA in individual cells, can capture rapid responses in the adult zebrafish heart after injury. Within the first 6 h, we detect activation of innate immune programs, including Toll-like receptor signaling, in a subset of macrophage-like immune cells. Analysis of a larger single-cell dataset indicates that neutrophils also contribute to this early response. Guided by these data, we show that macrophage-specific inhibition of the Toll-like receptor adaptor MyD88 reduces the pro-inflammatory macrophage response at the injury site and improves early hallmarks of regeneration. Our work establishes RNA metabolic labeling as a useful approach for measuring acute responses in vivo at single-cell resolution and identifies early immune-cell activation as a tunable component of heart regeneration.
Descrizione del documento:Online veröffentlicht: 05. Mai 2026
Gesehen am 17.08.2026
Descrizione fisica:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-026-72781-2

Documenti analoghi