Superior 3-year kidney graft function in patients with impaired pretransplant Th2 responses
In a prospective study of 80 patients, we investigated the association of kidney graft rejection with pretransplant CD4 helper/suppressor function, B cell responses, and in vitro cytokine secretion. A pokeweed mitogen-driven allogeneic coculture system was used to assess CD4 helper/suppressor functi...
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| Auteurs principaux: | , , , , , |
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| Format: | Article (Journal) |
| Langue: | anglais |
| Publié: |
June 1998
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| In: |
Transplant international
Year: 1998, Volume: 11, Pages: 350-356 |
| ISSN: | 1432-2277 |
| DOI: | 10.1111/j.1432-2277.1998.tb01154.x |
| Accès en ligne: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.1111/j.1432-2277.1998.tb01154.x Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.1998.tb01154.x 
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| Notes sur l'auteur: | R. Weimer, S. Zipperle, V. Daniel, S. Carl, G. Staehler, G. Opelz |
| Résumé: | In a prospective study of 80 patients, we investigated the association of kidney graft rejection with pretransplant CD4 helper/suppressor function, B cell responses, and in vitro cytokine secretion. A pokeweed mitogen-driven allogeneic coculture system was used to assess CD4 helper/suppressor function and T cell-dependent B cell responses. SAC I was used for T cell- and monocyte-independent stimulation of B cell cultures. B cell differentiation was assessed in a reverse hemolytic plaque assay. ELISAs were used to determine in vitro cytokine secretion. None of the 12 patients with pretransplant CD4 helper defects (< 10% helper activity) had acute rejection episodes in contrast to 32 of 68 (47%) patients with normal pretransplant CD4 helper function (P = 0.001). Patients with pretransplant CD4 helper defects exhibited better 3-year graft function than patients without CD4 helper defects (serum creatinine of functioning grafts: 1.2 +/- 0.1 mg/dl compared to 1.7 +/- 0.1 mg/dl, P < 0.05). Low pretransplant IL-10 responses (< 100 pg/ml; 14/80 patients) were significantly associated with a low incidence of acute rejection episodes (P < 0.01) and good 3-year graft function (P < 0.05). These data show that impaired pretransplant Th2 responses-CD4 help and IL-10 responses-predict a low risk of kidney graft rejection and good 3-year graft function, whereas Th1 (IL-2, IFN-gamma) and B cell/monocyte responses are not of predictive value. |
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| Description: | Gesehen am 26.08.2026 |
| Description matérielle: | Online Resource |
| ISSN: | 1432-2277 |
| DOI: | 10.1111/j.1432-2277.1998.tb01154.x |