Deadenylation and translation regulation of nanos mRNA in Drosophila embryo extract: [kumulative Dissertation]
Drosophila; Embryonalentwicklung; in vitro; Translationsregulation; Deadenylierung; nanos-mRNA; Smaug; Oskar
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| Dokumenttyp: | Book/Monograph Hochschulschrift |
| Sprache: | Englisch |
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2010
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| Verfasserangaben: | von Mandy Jeske |
MARC
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| 245 | 1 | 0 | |a Deadenylation and translation regulation of nanos mRNA in Drosophila embryo extract |b [kumulative Dissertation] |c von Mandy Jeske |
| 264 | 1 | |c 2010 | |
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| 500 | |a Tag der Verteidigung: 06.12.2010 | ||
| 502 | |a Halle, Univ., Naturwissenschaftliche Fakultät I, Diss., 2010 | ||
| 520 | |a Drosophila; Embryonalentwicklung; in vitro; Translationsregulation; Deadenylierung; nanos-mRNA; Smaug; Oskar | ||
| 520 | |a Drosophila; embryonic development; in vitro; translational regulation; deadenylation; nanos mRNA; Smaug; Oskar | ||
| 520 | |a Während der frühen Embryonalentwicklung von Drosophila wird die Genexpression hauptsächlich auf der Ebene der Translation reguliert. Die nanos-mRNA determiniert die Entwicklung der posterioren Strukturen des Drosophila-Embryos. Die nanos-mRNA wird nur am posterioren Pol des Embryos translatiert, während der größte Anteil der mRNA, der sich im Zytoplasma des Embryos befindet, inaktiv verbleibt. Die Translationsrepression der nanos-mRNA wird durch das Protein Smaug vermittelt, welches an die Smaug Recognition Elements (SREs) im nanos-3’ UTR binden kann. Am posterioren Pol führt das Oskar-Protein zur Aufhebung der Repression der nanos-mRNA. In dieser Doktorarbeit wurde ein In vitro-System aus Drosophila-Embryonen etabliert, dass die SRE-abhängige Regulation der nanos-mRNA rekapituliert. Die SREs vermitteln sowohl eine Deadenylierung als auch eine Translationsrepression von Reporter-RNAs. Die Zugabe von rekombinantem Oskar-Protein zum Extrakt verhindert die SRE-abhängige Repression. | ||
| 520 | |a During early embryogenesis of Drosophila, transcription does not contribute to the regulation of gene expression, and translational control becomes eminently important. The nanos mRNA specifies the development of posterior structures in the Drosophila embryo. Translation of nanos mRNA is restricted to the posterior pole of the embryo, and the vast majority of nanos mRNA within the embryonic cytoplasm is silenced. Translational repression of nanos is mediated by the protein Smaug, which can bind specific sequences, Smaug recognition elements (SREs), in the nanos 3’ UTR. At the posterior pole, translation of nanos mRNA is derepressed by the action of Oskar protein. In this doctoral study, a cell-free system derived from Drosophila embryos was established that recapitulates the SRE-dependent translational regulation. Both SRE-dependent deadenylation and translation repression were biochemically investigated. Addition of recombinant Oskar to the extracts prevents SRE-dependent repression. | ||
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| 700 | 1 | |a Hüttelmaier, Stefan |c Prof. Dr. |e Gutachter |4 oth | |
| 700 | 1 | |a Hentze, Matthias |c Prof. Dr. |e Gutachter |4 oth | |
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