Antiviral methods and protocols

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Bibliographic Details
Other Authors: Gong, Edwin Yunhao (Editor)
Format: Edited Volume
Language:English
Published: New York [u.a.] Humana Press c2013
Edition:2. ed.
Series:Methods in molecular biology 1030
In: Methods in molecular biology (1030)

Volumes / Articles: Show Volumes / Articles.
Subjects:
Online Access:Verlag, Inhaltsverzeichnis, Inhaltsverzeichnis: http://www.gbv.de/dms/bs/toc/75626426X.pdf
Cover: https://swbplus.bsz-bw.de/bsz392312670cov.jpg
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Author Notes:edited by Edwin Yunhao Gong
Table of Contents:
  • Part IHuman immunodeficiency virus type 1 (HIV-1)Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors Peggy Geluykens ... [et al.]
  • Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion Liesbet Smeulders ... [et al.]
  • Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay Bertrand Van Schoubroeck ... [et al.]
  • Biochemical screening assays to identify HIV-1 integrase inhibitors Marleen Clynhens ... [et al.]
  • HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance Peter Van den Eede ... [et al.]
  • Part IIHepatitis virusesIn vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis Rumin Zhang and William T. Windsor
  • Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template Eric Ferrari and Hsueh-Cheng Huang
  • Selecting and characterizing drug-resistant hepatitis C virus replicon Inge Vliegen, Leen Delang, and Johan Neyts
  • Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors Leen Vijgen ... [et al.]
  • Expression and purification of hepatitis C virus protease from clinical samples Liesbet van der Helm
  • Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase Erna Cleiren, Benoit Devogelaere, and Katleen Fierens
  • Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b Diana Koletzki ... [et al.]
  • Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures Dawei Cai ... [et al.]
  • In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates Yang Liu and Kathryn M. Kitrinos
  • Part IIIFlavivirusesCell-based antiviral assays for screening and profiling inhibitors against dengue virus Edwin Yunhao Gong ... [et al.]
  • Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes Edwin Yunhao Gong ... [et al.]
  • High-throughput screening using dengue virus reporter genomes Wolfgang Fischl and Ralf Bartenschlager
  • Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate Christoph Nitsche and Christian D. Klein
  • Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase Edwin Yunhao Gong ... [et al.]
  • Detection and quantification of flavivirus NS5 methyl- transferase activities Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi
  • Testing antiviral compounds in a dengue mouse model Wouter Schul, Andy Yip, and Pei-Yong Shi
  • Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan
  • Part IVHerpes virusesMethods for screening and profiling inhibitors of herpes simplex viruses Edwin Yunhao Gong
  • In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models Frances Valencia, Ronald L. Veselenak, and Nigel Bourne
  • Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors Christel Van den Eynde ... [et al.]
  • Part VRespiratory virusesFluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus Leen Kwanten, Ben De Clerck, and Dirk Roymans
  • Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells Anna Lundin, Tomas Bergström, and Edward Trybala
  • Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models Joke Van den Berg, Leen Kwanten, and Dirk Roymans
  • In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model Lieselot Houspie ... [et al.]
  • Methods to determine mechanism of action of anti-influenza inhibitors Angela Luttick, Stephanie Hamilton, and Simon P. Tucker
  • Methods for evaluation of antiviral efficacy against influenza virus infections in animal models Donald F. Smee and Dale L. Barnard
  • Part VIAlphavirusesDevelopment of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell Edwin Yunhao Gong ... [et al.]
  • Mouse model of chikungunya virus with utility in antiviral studies Ashley Dagley and Justin G. Julander.