NF135.C10: a new Plasmodium falciparum clone for controlled human malaria infections

We established a new field clone of Plasmodium falciparum for use in controlled human malaria infections and vaccine studies to complement the current small portfolio of P. falciparum strains, primarily based on NF54. The Cambodian clone NF135.C10 consistently produced gametocytes and generated subs...

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Main Authors: Teirlinck, Anne C. (Author) , Roestenberg, Meta (Author) , Vegte-Bolmer, Marga van de (Author)
Other Authors: Scholzen, Anja (Other) , Heinrichs, Moniek J. L. (Other) , Siebelink-Stoter, Rianne (Other) , Graumans, Wouter (Other) , Gemert, Geert-Jan van (Other) , Teelen, Karina (Other) , Vos, Martijn W. (Other) , Nganou-Makamdop, Krystelle (Other) , Borrmann, Steffen (Other) , Rozier, Yolanda P. A. (Other) , Erkens, Marianne A. A. (Other) , Luty, Adrian J. F. (Other) , Hermsen, Cornelus Christianus (Other) , Sim, B. Kim Lee (Other) , Lieshout, Lisette van (Other) , Hoffman, Stephen L. (Other) , Visser, Leo G. (Other) , Sauerwein, Robert W. (Other)
Format: Article (Journal)
Language:English
Published: 2013
In: The journal of infectious diseases
Year: 2013, Volume: 207, Issue: 4, Pages: 656-660
ISSN:1537-6613
DOI:10.1093/infdis/jis725
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/infdis/jis725
Verlag, Abstract, lizenzpflichtig, Volltext: http://jid.oxfordjournals.org/content/207/4/656.abstract
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Author Notes:Anne C. Teirlinck, Meta Roestenberg, Marga van de Vegte-Bolmer, Anja Scholzen, Moniek J.L. Heinrichs, Rianne Siebelink-Stoter, Wouter Graumans, Geert-Jan van Gemert, Karina Teelen, Martijn W. Vos, Krystelle Nganou-Makamdop, Steffen Borrmann, Yolanda P.A. Rozier, Marianne A.A. Erkens, Adrian J.F. Luty, Cornelus C. Hermsen, B. Kim Lee Sim, Lisette van Lieshout, Stephen L. Hoffman, Leo G. Visser, and Robert W. Sauerwein
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Summary:We established a new field clone of Plasmodium falciparum for use in controlled human malaria infections and vaccine studies to complement the current small portfolio of P. falciparum strains, primarily based on NF54. The Cambodian clone NF135.C10 consistently produced gametocytes and generated substantial numbers of sporozoites in Anopheles mosquitoes and diverged from NF54 parasites by genetic markers. In a controlled human malaria infection trial, 3 of 5 volunteers challenged by mosquitoes infected with NF135.C10 and 4 of 5 challenged with NF54 developed parasitemia as detected with microscopy. The 2 strains induced similar clinical signs and symptoms as well as cellular immunological responses.
Item Description:Electronically published 27 November 2012
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Physical Description:Online Resource
ISSN:1537-6613
DOI:10.1093/infdis/jis725