Buchumschlag

Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells

Previous studies underlined the capacity of recombinant yeast as efficient vehicle for the targeted delivery of functional nucleic acids as well as proteinaceous antigens to mammalian antigen-presenting cells (APCs). To improve this yeast-mediated cargo transport into APCs, we investigated the impac...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Walch-Rückheim, Barbara (VerfasserIn) , Kiefer, Ruth Annabelle (VerfasserIn) , Geginat, Gernot (VerfasserIn) , Schmitt, Manfred J. (VerfasserIn) , Breinig, Frank (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2016
In: Gene therapy
Year: 2015, Jahrgang: 23, Heft: 1, Pages: 103-107
ISSN:1476-5462
DOI:10.1038/gt.2015.77
Online-Zugang:Verlag, Volltext: https://doi.org/10.1038/gt.2015.77
Verlag, Volltext: https://www.nature.com/articles/gt201577
Verlag, Abstract, Volltext: http://www.nature.com/gt/journal/v23/n1/abs/gt201577a.html
Volltext
Verfasserangaben:B. Walch-Rückheim, R. Kiefer, G. Geginat, M.J. Schmitt and F. Breinig

MARC

LEADER 00000caa a2200000 c 4500
001 839818653
003 DE-627
005 20240130194541.0
007 cr uuu---uuuuu
008 151117r20162015xx |||||o 00| ||eng c
024 7 |a 10.1038/gt.2015.77  |2 doi 
035 |a (DE-627)839818653 
035 |a (DE-576)9839818651 
035 |a (DE-599)GBV839818653 
035 |a (OCoLC)1340144912 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Walch-Rückheim, Barbara  |d 1981-  |e VerfasserIn  |0 (DE-588)140158987  |0 (DE-627)616452268  |0 (DE-576)314838376  |4 aut 
245 1 0 |a Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells  |c B. Walch-Rückheim, R. Kiefer, G. Geginat, M.J. Schmitt and F. Breinig 
264 1 |c 2016 
300 |a 5 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Published online 20 August 2015 
500 |a Gesehen am 13.06.2019 
520 |a Previous studies underlined the capacity of recombinant yeast as efficient vehicle for the targeted delivery of functional nucleic acids as well as proteinaceous antigens to mammalian antigen-presenting cells (APCs). To improve this yeast-mediated cargo transport into APCs, we investigated the impact of coexpression of the human membrane-perturbing protein perforin in comparison with bacterial listeriolysin O (LLO) on the yeast-based delivery of DNA, mRNA and proteins to mammalian APCs. In contrast to LLO, a cholesterol-dependent pore-forming toxin of Listeria, intracellular expression of human perforin in Saccharomyces cerevisiae had no impact on yeast cell viability, while its coexpression significantly increased translocation of ovalbumin and subsequent activation of ovalbumin-specific T lymphocytes. Likewise, perforin improved the expression of the model antigen enhanced green fluorescent protein after yeast-mediated DNA and mRNA delivery, whereas LLO was only able to enhance DNA delivery. Taken together, our data show that human perforin, besides bacterial hemolysins, represents a promising means to improve the yeast-mediated delivery of functional nucleic acids and proteins to mammalian APCs. 
534 |c 2015 
700 1 |a Kiefer, Ruth Annabelle  |d 1975-  |e VerfasserIn  |0 (DE-588)124479855  |0 (DE-627)363375740  |0 (DE-576)294190899  |4 aut 
700 1 |a Geginat, Gernot  |e VerfasserIn  |0 (DE-588)1050911725  |0 (DE-627)785281142  |0 (DE-576)40535553X  |4 aut 
700 1 |a Schmitt, Manfred J.  |e VerfasserIn  |4 aut 
700 1 |a Breinig, Frank  |e VerfasserIn  |4 aut 
773 0 8 |i Enthalten in  |t Gene therapy  |d London : Nature Publ. Group, 1997  |g 23(2016), 1, Seite 103-107  |h Online-Ressource  |w (DE-627)306583623  |w (DE-600)1497870-2  |w (DE-576)094144656  |x 1476-5462  |7 nnas  |a Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells 
773 1 8 |g volume:23  |g year:2016  |g number:1  |g pages:103-107  |g extent:5  |a Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells 
856 4 0 |u https://doi.org/10.1038/gt.2015.77  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u https://www.nature.com/articles/gt201577  |x Verlag  |3 Volltext 
856 4 0 |u http://www.nature.com/gt/journal/v23/n1/abs/gt201577a.html  |x Verlag  |y Abstract  |3 Volltext 
951 |a AR 
992 |a 20190613 
993 |a Article 
994 |a 2016 
998 |g 1050911725  |a Geginat, Gernot  |m 1050911725:Geginat, Gernot  |d 60000  |d 63200  |e 60000PG1050911725  |e 63200PG1050911725  |k 0/60000/  |k 1/60000/63200/  |p 3 
999 |a KXP-PPN839818653  |e 3486834258 
BIB |a Y 
SER |a journal 
JSO |a {"physDesc":[{"extent":"5 S."}],"relHost":[{"id":{"zdb":["1497870-2"],"eki":["306583623"],"issn":["1476-5462"]},"origin":[{"publisherPlace":"London ; London [u.a.]","dateIssuedDisp":"1997-","dateIssuedKey":"1997","publisher":"Nature Publ. Group ; Macmillan"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Gene therapy","title":"Gene therapy"}],"part":{"extent":"5","text":"23(2016), 1, Seite 103-107","volume":"23","pages":"103-107","issue":"1","year":"2016"},"pubHistory":["Nachgewiesen 4.1997 -"],"recId":"306583623","language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cellsGene therapy","note":["Gesehen am 20.11.18"]}],"origin":[{"dateIssuedDisp":"2016","dateIssuedKey":"2016"}],"id":{"doi":["10.1038/gt.2015.77"],"eki":["839818653"]},"name":{"displayForm":["B. Walch-Rückheim, R. Kiefer, G. Geginat, M.J. Schmitt and F. Breinig"]},"note":["Published online 20 August 2015","Gesehen am 13.06.2019"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"recId":"839818653","title":[{"title":"Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells","title_sort":"Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells"}],"person":[{"given":"Barbara","family":"Walch-Rückheim","role":"aut","display":"Walch-Rückheim, Barbara","roleDisplay":"VerfasserIn"},{"family":"Kiefer","given":"Ruth Annabelle","display":"Kiefer, Ruth Annabelle","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Geginat","given":"Gernot","display":"Geginat, Gernot","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Manfred J.","family":"Schmitt","role":"aut","display":"Schmitt, Manfred J.","roleDisplay":"VerfasserIn"},{"given":"Frank","family":"Breinig","role":"aut","roleDisplay":"VerfasserIn","display":"Breinig, Frank"}]} 
SRT |a WALCHRUECKCOEXPRESSI2016 

Ähnliche Einträge