Regionally distinct regulation of astroglial neurotransmitter receptors by fibroblast growth factor-2
Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19-30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-rece...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
25 May 2002
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| In: |
Molecular and cellular neuroscience
Year: 2000, Volume: 16, Issue: 1, Pages: 42-58 |
| ISSN: | 1095-9327 |
| DOI: | 10.1006/mcne.2000.0857 |
| Online Access: | Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1044743100908571 Verlag, Volltext: http://dx.doi.org/10.1006/mcne.2000.0857 
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| Author Notes: | Bernhard Reuss, Doreen S.Y. Leung, Carsten Ohlemeyer, Helmut Kettenmann, and Klaus Unsicker |
| Summary: | Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19-30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]i. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. These results indicate that downregulation of astroglial gap junctions by FGF-2 is accompanied by an upregulation of D1DR and DA sensitivity, adding a new aspect to the role of FGF-2 in the regulation of brain functions. |
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| Item Description: | Gesehen am 24.11.2016 |
| Physical Description: | Online Resource |
| ISSN: | 1095-9327 |
| DOI: | 10.1006/mcne.2000.0857 |