Hepatitis B virus-infected HepG2hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8+ T cells in vitro
CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HB...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 July 2015
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| In: |
Journal of virology
Year: 2015, Volume: 89, Issue: 14, Pages: 7433-7438 |
| ISSN: | 1098-5514 |
| DOI: | 10.1128/JVI.00605-15 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1128/JVI.00605-15 Verlag, kostenfrei, Volltext: https://journals.asm.org/doi/10.1128/jvi.00605-15 
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| Author Notes: | Alexander Hoh, Maximilian Heeg, Yi Ni, Anita Schuch, Benedikt Binder, Nadine Hennecke, Hubert E. Blum, Michael Nassal, Ulrike Protzer, Maike Hofmann, Stephan Urban, Robert Thimme |
| Summary: | CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells. This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral loads. |
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| Item Description: | Im Titel ist „hNTCP“ und "+"hochgestellt Gesehen am 04.04.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1098-5514 |
| DOI: | 10.1128/JVI.00605-15 |