The roots of plant defenses: integrative multivariate analyses uncover dynamic behaviors of gene and metabolic networks of roots elicited by leaf herbivory
High-throughput analyses have frequently been used to characterize herbivory-induced reconfigurations in plant primary and secondary metabolism in above- and below-ground tissues, but the conclusions drawn from these analyses are often limited by the univariate methods used to analyze the data. Here...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 January 2014
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| In: |
The plant journal
Year: 2014, Volume: 77, Issue: 6, Pages: 880-892 |
| ISSN: | 1365-313X |
| DOI: | 10.1111/tpj.12439 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1111/tpj.12439 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12439/abstract 
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| Author Notes: | Jyotasana Gulati, Ian T. Baldwin and Emmanuel Gaquerel |
| Summary: | High-throughput analyses have frequently been used to characterize herbivory-induced reconfigurations in plant primary and secondary metabolism in above- and below-ground tissues, but the conclusions drawn from these analyses are often limited by the univariate methods used to analyze the data. Here we use our previously described multivariate time-series data analysis to evaluate leaf herbivory-elicited transcriptional and metabolic dynamics in the roots of Nicotiana attenuata. We observed large, but transient, systemic responses in the roots that contrasted with the pattern of co-linearity observed in the up- and downregulation of genes and metabolites across the entire time series in treated and systemic leaves. Using this newly developed approach for the analysis of whole-plant molecular responses in a time-course multivariate data set, we simultaneously analyzed stress responses in leaves and roots in response to the elicitation of a leaf. We found that transient systemic responses in roots resolved into two principal trends characterized by: (i) an inversion of root-specific semi-diurnal (12 h) transcript oscillations and (ii) transcriptional changes with major amplitude effects that translated into a distinct suite of root-specific secondary metabolites (e.g. alkaloids synthesized in the roots of N. attenuata). These findings underscore the importance of understanding tissue-specific stress responses in the correct day-night phase context and provide a holistic framework for the important role played by roots in above-ground stress responses. |
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| Item Description: | Gesehen am 08.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1365-313X |
| DOI: | 10.1111/tpj.12439 |