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The Glycoprotein NOWA and Minicollagens Are Part of a Disulfidelinked Polymer That Forms the Cnidarian Nematocyst Wall

The nematocyst is a unique extrusive organelle involved in the defense and capture of prey in cnidarians. Minicollagens and the glycoprotein NOWA are major components of the nematocyst capsule wall, which resists osmotic pressure of 15 MPa. Here we present the recombinant expression of NOWA, which s...

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Bibliographic Details
Main Authors: Özbek, Suat (Author) , Holstein, Thomas W. (Author)
Format: Article (Journal)
Language:English
Published: October 4, 2004,
In: Plant physiology
Year: 2004, Volume: 279, Issue: 50, Pages: 52016-52023
ISSN:1532-2548
DOI:10.1074/jbc.M407613200
Online Access:Verlag, Volltext: http://dx.doi.org/10.1074/jbc.M407613200
Verlag, Volltext: http://www.jbc.org/content/279/50/52016
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Author Notes:Suat Özbek, Elena Pokidysheva, Martine Schwager, Therese Schulthess, Naushaba Tariq, Dirk Barth, Alexander G. Milbradt, Luis Moroder, Jürgen Engel, and Thomas W. Holstein
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Summary:The nematocyst is a unique extrusive organelle involved in the defense and capture of prey in cnidarians. Minicollagens and the glycoprotein NOWA are major components of the nematocyst capsule wall, which resists osmotic pressure of 15 MPa. Here we present the recombinant expression of NOWA, which spontaneously assembles to globular macromolecular particles that are sensitive to reduction as the native wall structure. Ultra-structural analysis showed that the Hydra nematocyst wall is composed of several layers of globular particles, which are interconnected via radiating rodlike protrusions. Evidence is presented that native wall particles contain NOWA and minicollagen, supposed to be linked via disulfide bonds between their homologous cysteine-rich domains. Our data suggest a continuous suprastructure of the nematocyst wall, assembled from wall proteins that share a common oligomerization motif.
Item Description:Gesehen am 09.05.2017
Physical Description:Online Resource
ISSN:1532-2548
DOI:10.1074/jbc.M407613200

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