Phosphorylation of ZEN-4/MKLP1 by Aurora B regulates completion of cytokinesis
Summary The central spindle regulates the formation and positioning of the contractile ring and is essential for completion of cytokinesis [1]. Central spindle assembly begins in early anaphase with the bundling of overlapping, antiparallel, nonkinetochore microtubules [2, 3], and these bundles beco...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
April 26, 2005
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| In: |
Current biology
Year: 2005, Volume: 15, Issue: 8, Pages: 778-786 |
| ISSN: | 1879-0445 |
| DOI: | 10.1016/j.cub.2005.03.041 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.cub.2005.03.041 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0960982205003350 
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| Author Notes: | Annika Guse, Masanori Mishima and Michael Glotzer |
| Summary: | Summary The central spindle regulates the formation and positioning of the contractile ring and is essential for completion of cytokinesis [1]. Central spindle assembly begins in early anaphase with the bundling of overlapping, antiparallel, nonkinetochore microtubules [2, 3], and these bundles become compacted and mature into the midbody. Prominent components of the central spindle include aurora B kinase and centralspindlin, a complex containing a Kinesin-6 protein (ZEN-4/MKLP1) and a Rho family GAP (CYK-4/MgcRacGAP) that is essential for central spindle assembly [4]. Centralspindlin localization depends on aurora B kinase [5]. Aurora B concentrates in the midbody and persists between daughter cells. Here, we show that in C. elegans embryos and in cultured human cells, respectively, ZEN-4 and MKLP1 are phosphorylated by aurora B in vitro and in vivo on conserved C-terminal serine residues. In C. elegans embryos, a nonphosphorylatable mutant of ZEN-4 localizes properly but does not efficiently support completion of cytokinesis. In mammalian cells, an inhibitor of aurora kinase acutely attenuates phosphorylation of MKLP1. Inhibition of aurora B in late anaphase causes cytokinesis defects without disrupting the central spindle. These data indicate a conserved role for aurora-B-mediated phosphorylation of ZEN-4/MKLP1 in the completion of cytokinesis. |
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| Item Description: | Gesehen am 10.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0445 |
| DOI: | 10.1016/j.cub.2005.03.041 |