CENP-A confers a reduction in height on octameric nucleosomes
Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octame...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
5 May 2013
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| In: |
Nature structural & molecular biology
Year: 2013, Volume: 20, Issue: 6, Pages: 763-765 |
| ISSN: | 1545-9985 |
| DOI: | 10.1038/nsmb.2574 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1038/nsmb.2574 Verlag, Volltext: https://www.nature.com/nsmb/journal/v20/n6/full/nsmb.2574.html#affil-auth 
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| Author Notes: | Matthew D. D. Miell, Colin J. Fuller, Annika Guse, Helena M. Barysz, Andrew Downes, Tom Owen-Hughes, Juri Rappsilber, Aaron F. Straight & Robin C. Allshire |
| Summary: | Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes. |
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| Item Description: | Gesehen am 10.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1545-9985 |
| DOI: | 10.1038/nsmb.2574 |