Cep164 mediates vesicular docking to the mother centriole during early steps of ciliogenesis
Cilia formation is a multi-step process that starts with the docking of a vesicle at the distal part of the mother centriole. This step marks the conversion of the mother centriole into the basal body, from which axonemal microtubules extend to form the ciliary compartment. How vesicles are stably a...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 17, 2012
|
| In: |
The journal of cell biology
Year: 2012, Volume: 199, Issue: 7, Pages: 1083-1101 |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.201202126 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1083/jcb.201202126 Verlag, kostenfrei, Volltext: http://jcb.rupress.org/content/199/7/1083 
|
| Author Notes: | Kerstin N. Schmidt, Stefanie Kuhns, Annett Neuner, Birgit Hub, Hanswalter Zentgraf, and Gislene Pereira |
| Summary: | Cilia formation is a multi-step process that starts with the docking of a vesicle at the distal part of the mother centriole. This step marks the conversion of the mother centriole into the basal body, from which axonemal microtubules extend to form the ciliary compartment. How vesicles are stably attached to the mother centriole to initiate ciliary membrane biogenesis is unknown. Here, we investigate the molecular role of the mother centriolar component Cep164 in ciliogenesis. We show that Cep164 was indispensable for the docking of vesicles at the mother centriole. Using biochemical and functional assays, we identified the components of the vesicular transport machinery, the GEF Rabin8 and the GTPase Rab8, as interacting partners of Cep164. We propose that Cep164 is targeted to the apical domain of the mother centriole to provide the molecular link between the mother centriole and the membrane biogenesis machinery that initiates cilia formation. |
|---|---|
| Item Description: | Gesehen am 11.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.201202126 |