Genetic subtraction profiling identifies genes essential for Arabidopsisreproduction and reveals interaction between the female gametophyte and the maternal sporophyte
The embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtra...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
03 October 2007
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| In: |
Genome biology
Year: 2007, Volume: 8 |
| ISSN: | 1474-760X |
| DOI: | 10.1186/gb-2007-8-10-r204 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1186/gb-2007-8-10-r204
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| Author Notes: | Amal J. Johnston, Patrick Meier, Jacqueline Gheyselinck, Samuel E.J. Wuest, Michael Federer, Edith Schlagenhauf, Jörg D. Becker, Ueli Grossniklaus |
| Summary: | The embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant lacking an embryo sac in order to identify embryo sac expressed genes in this model organism. The influences of the embryo sac on the surrounding sporophytic tissues were previously thought to be negligible or nonexistent; we investigated the extent of these interactions by transcriptome analysis. |
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| Item Description: | Gesehen am 11.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1474-760X |
| DOI: | 10.1186/gb-2007-8-10-r204 |