Methods for generating year-round access to amphioxus in the laboratory
Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphio...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 26, 2013
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| In: |
PLOS ONE
Year: 2013, Volume: 8, Issue: 8, Pages: e71599 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0071599 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1371/journal.pone.0071599 Verlag, Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071599 
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| Author Notes: | Èlia Benito-Gutiérrez, Hermann Weber, Diana Virginia Bryant, Detlev Arendt |
| Summary: | Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphioxus colonies in the laboratory and obtaining embryos at any time of day and over almost the entire year, far exceeding natural conditions. This is achieved by mimicking the natural benthic environment, natural day- and moon- light, natural substrate and by providing a strictly controlled and seasonally fluctuating temperature regimen. Moreover, supplemented algae diets allow animals to refill their gonads in consecutive years. Spontaneous spawning, a major problem in previous setups, no longer occurs in our facility; instead, all breeding is induced and fertilization occurs fully in vitro. Our system makes amphioxus a standard laboratory animal model. |
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| Item Description: | Gesehen am 12.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0071599 |