Large-scale analysis of the regulatory architecture of the mouse genome with a transposon-associated sensor
We present here a Sleeping Beauty-based transposition system that offers a simple and efficient way to investigate the regulatory architecture of mammalian chromosomes in vivo. With this system, we generated several hundred mice and embryos, each with a regulatory sensor inserted at a random genomic...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 March 2011
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| In: |
Nature genetics
Year: 2011, Volume: 43, Issue: 4, Pages: 379-386 |
| ISSN: | 1546-1718 |
| DOI: | 10.1038/ng.790 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1038/ng.790 Verlag, Volltext: https://www.nature.com/ng/journal/v43/n4/full/ng.790.html 
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| Author Notes: | Sandra Ruf, Orsolya Symmons, Veli Vural Uslu, Dirk Dolle, Chloé Hot, Laurence Ettwiller, François Spitz |
| Summary: | We present here a Sleeping Beauty-based transposition system that offers a simple and efficient way to investigate the regulatory architecture of mammalian chromosomes in vivo. With this system, we generated several hundred mice and embryos, each with a regulatory sensor inserted at a random genomic position. This large sampling of the genome revealed the widespread presence of long-range regulatory activities along chromosomes, forming overlapping blocks with distinct tissue-specific expression potentials. The presence of tissue-restricted regulatory activities around genes with widespread expression patterns challenges the gene-centric view of genome regulation and suggests that most genes are modulated in a tissue-specific manner. The local hopping property of Sleeping Beauty provides a dynamic approach to map these regulatory domains at high resolution and, combined with Cre-mediated recombination, allows for the determination of their functions by engineering mice with specific chromosomal rearrangements. |
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| Item Description: | Gesehen am 17.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1546-1718 |
| DOI: | 10.1038/ng.790 |