Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology
* Zinc plays a central role in all living cells as a cofactor for enzymes and as a structural element enabling the adequate folding of proteins. In eukaryotic cells, metals are highly compartmentalized and chelated. Although essential to characterize the mechanisms of Zn2+ homeostasis, the measureme...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
23 December 2013
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| In: |
The new phytologist
Year: 2014, Volume: 202, Issue: 1, Pages: 198-208 |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12652 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1111/nph.12652 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/nph.12652/abstract 
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| Author Notes: | Viviane Lanquar, Guido Grossmann, Jan L. Vinkenborg, Maarten Merkx, Sébastien Thomine and Wolf B. Frommer |
| Summary: | * Zinc plays a central role in all living cells as a cofactor for enzymes and as a structural element enabling the adequate folding of proteins. In eukaryotic cells, metals are highly compartmentalized and chelated. Although essential to characterize the mechanisms of Zn2+ homeostasis, the measurement of free metal concentrations in living cells has proved challenging and the dynamics are difficult to determine. * Our work combines the use of genetically encoded Förster resonance energy transfer (FRET) sensors and a novel microfluidic technology, the RootChip, to monitor the dynamics of cytosolic Zn2+ concentrations in Arabidopsis root cells. * Our experiments provide estimates of cytosolic free Zn2+ concentrations in Arabidopsis root cells grown under sufficient (0.4 nM) and excess (2 nM) Zn2+ supply. In addition, monitoring the dynamics of cytosolic [Zn2+] in response to external supply suggests the involvement of high- and low-affinity uptake systems as well as release from internal stores. * In this study, we demonstrate that the combination of genetically encoded FRET sensors and microfluidics provides an attractive tool to monitor the dynamics of cellular metal ion concentrations over a wide concentration range in root cells. |
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| Item Description: | Gesehen am 22.05.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12652 |