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CSC-1: a subunit of the Aurora B kinase complex that binds to the Survivin-like protein BIR-1 and the Incenp-like protein ICP-1

The Aurora B kinase complex is a critical regulator of chromosome segregation and cytokinesis. In Caenorhabditis elegans, AIR-2 (Aurora B) function requires ICP-1 (Incenp) and BIR-1 (Survivin). In various systems, Aurora B binds to orthologues of these proteins. Through genetic analysis, we have ide...

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Bibliographic Details
Main Authors: Romano, Alper (Author) , Guse, Annika (Author)
Format: Article (Journal) Working Paper
Language:English
Published: April 21, 2003
In: The journal of cell biology
Year: 2003, Volume: 161, Issue: 2, Pages: 229-236
ISSN:1540-8140
DOI:10.1083/jcb.200207117
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1083/jcb.200207117
Verlag, kostenfrei, Volltext: http://jcb.rupress.org/content/161/2/229
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Author Notes:Alper Romano, Annika Guse, Ivica Krascenicova, Heinke Schnabel, Ralf Schnabel, and Michael Glotzer
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Summary:The Aurora B kinase complex is a critical regulator of chromosome segregation and cytokinesis. In Caenorhabditis elegans, AIR-2 (Aurora B) function requires ICP-1 (Incenp) and BIR-1 (Survivin). In various systems, Aurora B binds to orthologues of these proteins. Through genetic analysis, we have identified a new subunit of the Aurora B kinase complex, CSC-1. C. elegans embryos depleted of CSC-1, AIR-2, ICP-1, or BIR-1 have identical phenotypes. CSC-1, BIR-1, and ICP-1 are interdependent for their localization, and all are required for AIR-2 localization. In vitro, CSC-1 binds directly to BIR-1. The CSC-1/BIR-1 complex, but not the individual subunits, associates with ICP-1. CSC-1 associates with ICP-1, BIR-1, and AIR-2 in vivo. ICP-1 dramatically stimulates AIR-2 kinase activity. This activity is not stimulated by CSC-1/BIR-1, suggesting that these two subunits function as targeting subunits for AIR-2 kinase.
Item Description:Gesehen am 22.05.2017
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.200207117

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