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Effects of low seawater pH on the marine polychaete Platynereis dumerilii

An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA),...

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Bibliographic Details
Main Authors: Wäge, Janine (Author) , Larsson, Tomas (Author) , Simakov, Oleg (Author) , Arendt, Detlev (Author)
Format: Article (Journal)
Language:English
Published: 15 June 2015
In: Marine pollution bulletin
Year: 2015, Volume: 95, Issue: 1, Pages: 166-172
ISSN:1879-3363
DOI:10.1016/j.marpolbul.2015.04.027
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.marpolbul.2015.04.027
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0025326X15002295
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Author Notes:Janine Wäge, Jörg D. Hardege, Tomas A. Larsson, Oleg Simakov, Emma C. Chapman, Detlev Arendt, Jeanette M. Rotchell
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Summary:An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1h and up-regulated after 7days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1h was also observed, suggesting a shift in the CO2 and HCO3− balance. No change in CaM expression was detected after 7days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism.
Item Description:Gesehen am 09.06.2017
Physical Description:Online Resource
ISSN:1879-3363
DOI:10.1016/j.marpolbul.2015.04.027

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