Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture
Hox transcription factors regulate the morphogenesis along the anterior-posterior (A/P) body axis through the interaction with small cis-regulatory modules (CRMs) of their target gene, however so far very few Hox CRMs are known and have been analyzed in detail. In this study we have identified a new...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
7 October 2011
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| In: |
Biochemical and biophysical research communications
Year: 2011, Volume: 414, Issue: 4, Pages: 761-766 |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2011.09.154 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.bbrc.2011.09.154 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X11017827 
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| Author Notes: | Zongzhao Zhai, Xingke Yang, Ingrid Lohmann |
| Summary: | Hox transcription factors regulate the morphogenesis along the anterior-posterior (A/P) body axis through the interaction with small cis-regulatory modules (CRMs) of their target gene, however so far very few Hox CRMs are known and have been analyzed in detail. In this study we have identified a new Hox CRM, ct340, which guides the expression of the cell type specification gene cut (ct) in the posterior spiracle under the direct control of the Hox protein Abdominal-B (Abd-B). Using the ct340 enhancer activity as readout, an efficient cloning system to generate VP16 activation domain fusion protein was developed to unambiguously test protein-DNA interaction in Drosophila cell culture. By functionally dissecting the Abd-B protein, new features of Abd-B dependent target gene regulation were detected. Due to its easy adaptability, this system can be generally used to map functional domains within sequence-specific transcriptional factors in Drosophila cell culture, and thus provide preliminary knowledge of the protein functional domain structure for further in vivo analysis. |
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| Item Description: | Gesehen am 13.06.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2011.09.154 |