CRISPR genome engineering and viral gene delivery: a case of mutual attraction
The adaptation of the CRISPR/Cas9 DNA engineering machinery for mammalian cells has revolutionized our approaches to low- or high-throughput genome annotation and paved the way for conceptually novel therapeutic strategies. A large part of the attraction of CRISPR stems from the small size of its tw...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
6 February 2015
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| In: |
Biotechnology journal
Year: 2015, Volume: 10, Issue: 2, Pages: 258-272 |
| ISSN: | 1860-7314 |
| DOI: | 10.1002/biot.201400529 |
| Online Access: | Volltext Volltext 
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| Author Notes: | Florian Schmidt, Dirk Grimm |
| Summary: | The adaptation of the CRISPR/Cas9 DNA engineering machinery for mammalian cells has revolutionized our approaches to low- or high-throughput genome annotation and paved the way for conceptually novel therapeutic strategies. A large part of the attraction of CRISPR stems from the small size of its two core components - Cas9 and gRNA - and hence its compatibility with virtually any available viral vector delivery system. As a result, over the past two years, four major classes of viral vectors have already been engineered and applied as CRISPR delivery tools - retroviruses, lentiviruses, adenoviruses, and adeno-associated viruses (AAVs). The juxtaposition of these two technologies reflects a case of tremendous mutual attraction and holds unprecedented promises for biology and medicine. Here, we provide an overview of the state-of-the-art of this rapidly emerging field, from a comparative description of the principal vector designs, to a synopsis of some of the most exciting applications that were reported to date, including the use of viral CRISPR vectors for genome-wide loss-of-function screens, multiplexed gene editing or disease modeling in animals. Once specificity and safety have been improved further, viral vector-mediated in vitro/in vivo CRISPR delivery and expression promise to radically transform basic and applied biomedical research. |
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| Item Description: | Gesehen am 03.07.2017 |
| Physical Description: | Online Resource |
| ISSN: | 1860-7314 |
| DOI: | 10.1002/biot.201400529 |