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KOMA: ELISA-microarray calibration and data analysis based on kinetic signal amplification

Antibody microarrays with enzyme-linked immunosorbent technology are used for quantitative, simultaneous and high-throughput analysis of multiple proteins in a single probe. Kinetic detection can significantly improve precision and quantification range of microarray measurements. Here we present the...

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Bibliographic Details
Main Authors: Holenya, Pavlo (Author) , Heigwer, Florian (Author) , Wölfl, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 30 March 2012
In: Journal of immunological methods
Year: 2012, Volume: 380, Issue: 1/2, Pages: 10-15
ISSN:1872-7905
DOI:10.1016/j.jim.2012.03.006
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.jim.2012.03.006
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0022175912000853
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Author Notes:Pavlo Holenya, Florian Heigwer, Stefan Wölfl
Description
Summary:Antibody microarrays with enzyme-linked immunosorbent technology are used for quantitative, simultaneous and high-throughput analysis of multiple proteins in a single probe. Kinetic detection can significantly improve precision and quantification range of microarray measurements. Here we present the open source software Kinetic Operating Microarray Analyzer (KOMA) that enables calibration and high-throughput analysis of quantitative microarray data collected using a time-resolved kinetic detection protocol of the enzymatic signal. This tool can also be helpful for analyzing data from any other analytical assays employing enzymatic signal amplification, in which a broader range of quantification is reached by the time-resolved recording of readouts. KOMA is open for download at http://www.uni-heidelberg.de/fakultaeten/biowissenschaften/ipmb/biologie/woelfl/Research.html together with a set of test raw data and requires R version 2.12 and Java RE version 6.0.
Item Description:Gesehen am 07.07.2017
Deposit date: 2015-05-13
Physical Description:Online Resource
ISSN:1872-7905
DOI:10.1016/j.jim.2012.03.006

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