Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats
Objectives: This study was designed to evaluate the antihyperglycaemic activity of the methanol leaf extract of Eremophila maculata (EMM) both in vitro and in vivo. Methods: The antihyperglycaemic activity was assessed in vitro using differentiated 3T3-L1 adipocytes, whereas in-vivo effect was evalu...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
21 March 2017
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| In: |
Journal of pharmacy and pharmacology
Year: 2017, Volume: 69, Issue: 6, Pages: 733-742 |
| ISSN: | 2042-7158 |
| DOI: | 10.1111/jphp.12690 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1111/jphp.12690 Verlag, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/jphp.12690/abstract 
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| Author Notes: | Fadia S. Youssef, Mohamed L. Ashour, Sherif S. Ebada, Mansour Sobeh, Hesham A. El-Beshbishy, Abdel Nasser Singab and Michael Wink |
| Summary: | Objectives: This study was designed to evaluate the antihyperglycaemic activity of the methanol leaf extract of Eremophila maculata (EMM) both in vitro and in vivo. Methods: The antihyperglycaemic activity was assessed in vitro using differentiated 3T3-L1 adipocytes, whereas in-vivo effect was evaluated in streptozotocin-induced diabetic rats. Chemical profiling of EMM was done using LC-ESI-MS techniques. Molecular modelling experiments of the identified compounds were performed using C-Docker protocol. Key findings: Eremophila maculata slightly enhanced cellular glucose uptake and utilization in vitro by 3.92% relative to the untreated control. A stronger in-vivo effect was observed for EMM and its dichloromethane fraction. A pronounced elevation in serum insulin by 88.89 and 66.67%, respectively, accompanied by an apparent decline in fasting blood glucose (FBG) level by 65.60 and 70.37% comparable to streptozotocin-induced diabetic rats was observed. This effect was stronger than that of the reference drug glibenclamide (GLB). Chemical profiling of EMM revealed that leucoseptoside A, verbascoside, syringaresinol-4-O-β-D-glucopyranoside, pinoresinol-4-O-β-D-glucopyranoside and pinoresinol-4-O-[6″-O-(E)-feruloyl]-β-D-glucopyranoside are the major compounds. Molecular modelling showed that martynoside, verbascoside and phillygenin exhibited the highest inhibition to human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM) and aldose reductase (AR), respectively. Conclusion: Eremophila maculata offers an interesting relatively safer antihyperglycaemic candidate comparable to synthetic analogues. |
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| Item Description: | Gesehen am 28.07.2017 |
| Physical Description: | Online Resource |
| ISSN: | 2042-7158 |
| DOI: | 10.1111/jphp.12690 |