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Essential role of the V-ATPase in male gametophyte development

Intracellular pH homeostasis is a prerequisite for biological processes and requires the action of proton pumps. The vacuolar H+-ATPase (V-ATPase) is involved in regulating pH in endomembrane compartments of all eukaryotic cells. In plants, there is an additional endomembrane proton pump, H+-pyropho...

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Bibliographic Details
Main Authors: Dettmer, Jan (Author) , Schumacher, Karin (Author)
Format: Article (Journal)
Language:English
Published: 2005
In: The plant journal
Year: 2005, Volume: 41, Issue: 1, Pages: 117-124
ISSN:1365-313X
DOI:10.1111/j.1365-313X.2004.02282.x
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1111/j.1365-313X.2004.02282.x
Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2004.02282.x/abstract
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Author Notes:Jan Dettmer, Daniel Schubert, Olga Calvo-Weimar, York-Dieter Stierhof, Renate Schmidt and Karin Schumacher
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Summary:Intracellular pH homeostasis is a prerequisite for biological processes and requires the action of proton pumps. The vacuolar H+-ATPase (V-ATPase) is involved in regulating pH in endomembrane compartments of all eukaryotic cells. In plants, there is an additional endomembrane proton pump, H+-pyrophosphatase (H+-PPase). However, the relative roles of the two types of pumps in endomembrane acidification and energization of secondary active transport are unclear. Here, we show that a strong T-DNA insertion allele of VHA-A, the single copy gene encoding the catalytic subunit of the Arabidopsis V-ATPase, causes complete male and partial female gametophytic lethality. Severe changes in the morphology of Golgi stacks and Golgi-derived vesicles in male gametophytes are the first visible symptoms of cell degeneration leading to a failure to develop mature pollen. Similar effects on Golgi morphology were observed in pollen tubes when growth was blocked by Concanamycin A, a specific V-ATPase inhibitor. Taken together, our results suggests that V-ATPase function is essential for Golgi organization and development of the male gametophyte.
Item Description:Gesehen am 01.08.2017
Physical Description:Online Resource
ISSN:1365-313X
DOI:10.1111/j.1365-313X.2004.02282.x

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