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The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis

Precise control over organelle shapes is essential for cellular organization and morphogenesis. During yeast meiosis, prospore membranes (PSMs) constitute bell-shaped organelles that enwrap the postmeiotic nuclei leading to the cellularization of the mother cell's cytoplasm and to spore formati...

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Bibliographic Details
Main Authors: Maier, Peter (Author) , Maeder, Céline (Author) , Stelzer, Ernst H. K. (Author) , Knop, Michael (Author)
Format: Article (Journal)
Language:English
Published: 2008 Aug 28
In: The EMBO journal
Year: 2008, Volume: 27, Issue: 18, Pages: 2363-2374
ISSN:1460-2075
DOI:10.1038/emboj.2008.168
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/emboj.2008.168
Verlag, kostenfrei, Volltext: https://www.embopress.org/doi/full/10.1038/emboj.2008.168
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Author Notes:Peter Maier, Nicole Rathfelder, Celine I Maeder, Julien Colombelli, Ernst HK Stelzer and Michael Knop
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Summary:Precise control over organelle shapes is essential for cellular organization and morphogenesis. During yeast meiosis, prospore membranes (PSMs) constitute bell-shaped organelles that enwrap the postmeiotic nuclei leading to the cellularization of the mother cell's cytoplasm and to spore formation. Here, we analysed how the PSMs acquire their curved bell-shaped structure. We discovered that two antagonizing forces ensure PSM shaping and proper closure during cytokinesis. The Ssp1p-containing coat at the leading edge of the PSM generates a pushing force, which is counteracted by a novel pathway, the spore membrane-bending pathway (SpoMBe). Using genetics, we found that Sma2p and Spo1p, a phospholipase, as well as several GPI-anchored proteins belong to the SpoMBe pathway. They exert a force all along the membrane, responsible for membrane bending during PSM biogenesis and for PSM closure during cytokinesis. We showed that the SpoMBe pathway involves asymmetric distribution of Sma2p and does not involve a GPI-protein-containing matrix. Rather, repulsive forces generated by asymmetrically distributed and dynamically moving GPI-proteins are suggested as the membrane-bending principle.
Item Description:Gesehen am 14.08.2017
Physical Description:Online Resource
ISSN:1460-2075
DOI:10.1038/emboj.2008.168

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