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Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin

Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal...

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Main Authors: Höfner, Thomas (Author) , Macher-Göppinger, Stephan (Author) , Jauch, Anna (Author) , Weichert, Wilko (Author) , Pahernik, Sascha (Author)
Format: Article (Journal)
Language:English
Published: February 19, 2015
In: Stem cell reports
Year: 2015, Volume: 4, Issue: 3, Pages: 503-518
ISSN:2213-6711
DOI:10.1016/j.stemcr.2015.01.015
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.stemcr.2015.01.015
Verlag, kostenfrei, Volltext: http://www.cell.com/stem-cell-reports/abstract/S2213-6711(15)00036-3
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Author Notes:Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp
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Summary:Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin−SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin−CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.
Item Description:Gesehen am 30.08.2017
Physical Description:Online Resource
ISSN:2213-6711
DOI:10.1016/j.stemcr.2015.01.015

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