Imagen de Portada

Single-molecule FRET reveals the energy landscape of the full-length SAM-I riboswitch

S-adenosyl-L-methionine (SAM) ligand binding induces major structural changes in SAM-I riboswitches, through which gene expression is regulated via transcription termination. Little is known about the conformations and motions governing the function of the full-length Bacillus subtilis yitJ SAM-I ri...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Manz, Christoph (Autor) , Samanta, Ayan (Autor) , Jäschke, Andres (Autor) , Nienhaus, G. Ulrich (Autor)
Formato: Article (Journal)
Lenguaje:inglés
Publicado: 18 September 2017
In: Nature chemical biology
Year: 2017, Volumen: 13, Pages: 1172-1178
ISSN:1552-4469
DOI:10.1038/nchembio.2476
Acceso en línea:Verlag, Volltext: http://dx.doi.org/10.1038/nchembio.2476
Verlag, Volltext: https://www.nature.com/nchembio/journal/vaop/ncurrent/pdf/nchembio.2476.pdf
Verlag, Volltext: http://www.nature.com/nchembio/journal/vaop/ncurrent/full/nchembio.2476.html
Enlace del recurso
Notas de Autor:Christoph Manz, Andrei Yu Kobitski, Ayan Samanta, Bettina G. Keller, Andres Jäschke & G. Ulrich Nienhaus
Descripción
Sumario:S-adenosyl-L-methionine (SAM) ligand binding induces major structural changes in SAM-I riboswitches, through which gene expression is regulated via transcription termination. Little is known about the conformations and motions governing the function of the full-length Bacillus subtilis yitJ SAM-I riboswitch. Therefore, we have explored its conformational energy landscape as a function of Mg2+ and SAM ligand concentrations using single-molecule Förster resonance energy transfer (smFRET) microscopy and hidden Markov modeling analysis. We resolved four conformational states both in the presence and the absence of SAM and determined their Mg2+-dependent fractional populations and conformational dynamics, including state lifetimes, interconversion rate coefficients and equilibration timescales. Riboswitches with terminator and antiterminator folds coexist, and SAM binding only gradually shifts the populations toward terminator states. We observed a pronounced acceleration of conformational transitions upon SAM binding, which may be crucial for off-switching during the brief decision window before expression of the downstream gene.
Notas:Gesehen am 16.10.2017
Descripción Física:Online Resource
ISSN:1552-4469
DOI:10.1038/nchembio.2476

Ejemplares similares