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Impaired mitochondrial dynamics and mitophagy in neuronal models of tuberous sclerosis complex

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Tuberous Sclerosis Complex (TSC) is a neurodevelopmental disease caused by TSC1 or TSC2 mutations and subsequent activation of the mTORC1 kinase. Upon mTORC1 activation, anabolic metabolism, which requires mitochondria, is induced, yet at the same time the principal pathway for mitochondrial turnove...

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Main Authors: Ebrahimi-Fakhari, Darius (Author) , Saffari, Afshin (Author) , Wahlster, Lara (Author) , Kölker, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 25 October 2016
In: Cell reports
Year: 2016, Volume: 17, Issue: 4, Pages: 1053-1070
ISSN:2211-1247
DOI:10.1016/j.celrep.2016.09.054
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.celrep.2016.09.054
Verlag, kostenfrei, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078873/
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Author Notes:Darius Ebrahimi-Fakhari, Afshin Saffari, Lara Wahlster, Alessia Di Nardo, Daria Turner, Tommy L. Lewis, Christopher Conrad, Jonathan M. Rothberg, Jonathan O. Lipton, Stefan Kölker, Georg F. Hoffmann, Min-Joon Han, Franck Polleux, Mustafa Sahin
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Summary:Tuberous Sclerosis Complex (TSC) is a neurodevelopmental disease caused by TSC1 or TSC2 mutations and subsequent activation of the mTORC1 kinase. Upon mTORC1 activation, anabolic metabolism, which requires mitochondria, is induced, yet at the same time the principal pathway for mitochondrial turnover, autophagy, is compromised. How mTORC1 activation impacts mitochondrial turnover in neurons remains unknown. Here we demonstrate impaired mitochondrial homeostasis in neuronal in vitro and in vivo models of TSC. We find that Tsc1/2-deficient neurons accumulate mitochondria in cell bodies but are depleted of axonal mitochondria, including those supporting presynaptic sites. Axonal and global mitophagy of damaged mitochondria is impaired, suggesting that decreased turnover may act upstream of impaired mitochondrial metabolism. Importantly, blocking mTORC1 or inducing mTOR-independent autophagy restores mitochondrial homeostasis. Our study clarifies the complex relationship between the TSC-mTORC1 pathway, autophagy and mitophagy, and defines mitochondrial homeostasis as a therapeutic target for TSC and related diseases.,
Item Description:Gesehen am 06.11.2017
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2016.09.054

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