Impact of steroids on the inflammatory response after ischemic acute kidney injury in rats
Inflammation plays a crucial role in acute kidney injury (AKI). The current study was designed to analyze the influence of prednisolone treatment on the inflammatory reaction during the first 96 h after AKI induction in a rat model. AKI was induced by unilateral clipping of the renal vessels. The tr...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
31-Aug-2017
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| In: |
Indian journal of nephrology
Year: 2017, Volume: 27, Issue: 5, Pages: 365-371 |
| ISSN: | 1998-3662 |
| DOI: | 10.4103/ijn.IJN_40_17 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.4103/ijn.IJN_40_17 Verlag, Volltext: http://www.indianjnephrol.org/article.asp?issn=0971-4065;year=2017;volume=27;issue=5;spage=365;epage=371;aulast=Fontana;type=0 
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| Author Notes: | J. Fontana, A. Vogt, A. Hohenstein, U. Vettermann, E. Doroshenko, E. Lammer, B. A. Yard, S. Hoeger |
| Summary: | Inflammation plays a crucial role in acute kidney injury (AKI). The current study was designed to analyze the influence of prednisolone treatment on the inflammatory reaction during the first 96 h after AKI induction in a rat model. AKI was induced by unilateral clipping of the renal vessels. The treatment group received prednisolone 5 mg/kg s.c. daily. Infiltration rates of macrophages, leukocytes, and T-cells (24, 96 h) as well as plasma concentrations of the inflammatory markers intercellular adhesion molecule, interleukin-1 beta (IL-1β), IL-18, IL-6, and tumor necrosis factor-alpha (0, 6, 24, 96 h) were determined by fluorescence-activated cell sorting (FACS) analysis only. Ninety-six hours after AKI induction, the prednisolone group demonstrated significantly lower creatinine concentrations compared to the control group (<i>P</i> < 0.05). Twenty-four hours after induction of AKI, a significantly higher rate of infiltrating leukocytes was detectable with FACS analysis in the control group (<i>P</i> < 0.01) with a corresponding significantly higher rate of macrophages after 96 h (<i>P</i> < 0.01). IL-6 and IL-1β demonstrated a peak after 6 h with a significantly higher release in the control group (IL-6: <i>P</i> < 0.01; IL-1β: <i>P</i> < 0.05). In contrast to the control group, the prednisolone group demonstrated no further incline of IL-18 after 24 h. The results demonstrate the importance of stretching the observation period in an ischemia-reperfusion-induced AKI setting beyond the first 24 h. Despite the demonstrated protective effects of a continuous prednisolone application, it seems that this single anti-inflammatory agent will not be able to completely suppress the inflammatory response after an ischemia-reperfusion-induced AKI. |
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| Item Description: | Gesehen am 13.04.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1998-3662 |
| DOI: | 10.4103/ijn.IJN_40_17 |