Evaluation of third-generation ELISA and a rapid immunochromatographic assay for the detection of norovirus infection in fecal samples from inpatients of a German tertiary care hospital
The analytical accuracy of the RIDASCREEN Norovirus 3rd Generation ELISA assay and the rapid immunochromatographic RIDAQUICK Norovirus assay were determined in comparison to PCR. In a prospective study 410 consecutive samples were collected from inpatients of a tertiary care hospital in Germany. All...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
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| In: |
European journal of clinical microbiology & infectious diseases
Year: 2011, Volume: 31, Issue: 5, Pages: 733-737 |
| ISSN: | 1435-4373 |
| DOI: | 10.1007/s10096-011-1366-z |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s10096-011-1366-z Verlag, Volltext: https://link.springer.com/article/10.1007/s10096-011-1366-z 
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| Author Notes: | G. Geginat, D. Kaiser, S. Schrempf |
| Summary: | The analytical accuracy of the RIDASCREEN Norovirus 3rd Generation ELISA assay and the rapid immunochromatographic RIDAQUICK Norovirus assay were determined in comparison to PCR. In a prospective study 410 consecutive samples were collected from inpatients of a tertiary care hospital in Germany. All samples were tested with the two antigen detection assays, as well as with three different real-time reverse transcription PCR methods as the reference standard. A sample was considered true-positive if at least 2 out of 3 PCR methods yielded a positive signal (137 positive samples, >99% genogroup II). Compared with the PCR-based reference the overall diagnostic sensitivities of the ELISA and the immunochromatographic assay were 77% and 69% and the diagnostic specificities were 96% and 97% respectively. Both assays allow the rapid and economic screening of large numbers of samples and thus are useful diagnostic tools for the detection of suspected norovirus infections. |
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| Item Description: | Published online: 02 August 2011 Gesehen am 25.04.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1435-4373 |
| DOI: | 10.1007/s10096-011-1366-z |