Buchumschlag

In vivo analysis of centromeric proteins reveals a stem cell-specific asymmetry and an essential role in differentiated, non-proliferating cells

Summary Stem cells of the Drosophila midgut (ISCs) are the only mitotically dividing cells of the epithelium and, therefore, presumably the only epithelial cells that require functional kinetochores for microtubule spindle attachment during mitosis. The histone variant CENP-A marks centromeric chrom...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: García del Arco, Ana (VerfasserIn) , Edgar, Bruce (VerfasserIn) , Erhardt, Sylvia (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: February 20, 2018
In: Cell reports
Year: 2018, Jahrgang: 22, Heft: 8, Pages: 1982-1993
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.01.079
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.celrep.2018.01.079
Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124718301438
Volltext
Verfasserangaben:Ana García del Arco, Bruce A. Edgar, Sylvia Erhardt
Beschreibung
Zusammenfassung:Summary Stem cells of the Drosophila midgut (ISCs) are the only mitotically dividing cells of the epithelium and, therefore, presumably the only epithelial cells that require functional kinetochores for microtubule spindle attachment during mitosis. The histone variant CENP-A marks centromeric chromatin as the site of kinetochore formation and spindle attachment during mitotic chromosome segregation. Here, we show that centromeric proteins distribute asymmetrically during ISC division. Whereas newly synthesized CENP-A is enriched in differentiating progeny, CENP-C is undetectable in these cells. Remarkably, CENP-A persists in ISCs for weeks without being replaced, consistent with it being an epigenetic mark responsible for maintaining stem cell properties. Furthermore, CENP-A and its loading factor CAL1 were found to be essential for post-mitotic, differentiating cells; removal of any of these factors interferes with endoreduplication. Taken together, we propose two additional roles of CENP-A: to maintain stem cell-unique properties and to regulate post-mitotic cells.
Beschreibung:Gesehen am 30.04.2018
Beschreibung:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.01.079

Ähnliche Einträge