Crossreactivity of an antiserum directed to the gram-negative bacterium Neisseria gonorrhoeae with the SNARE-complex protein Snap23 correlates to impaired exocytosis in SH-SY5Y cells
Early maternal infections with Neisseria gonorrhoeae (NG) correlate to an increased lifetime schizophrenia risk for the offspring, which might be due to an immune-mediated mechanism. Here, we investigated the interactions of polyclonal antisera to NG (α-NG) with a first trimester prenatal brain mult...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
01 May 2017
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| In: |
Journal of molecular neuroscience
Year: 2017, Volume: 62, Issue: 2, Pages: 163-180 |
| ISSN: | 1559-1166 |
| DOI: | 10.1007/s12031-017-0920-2 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s12031-017-0920-2
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| Author Notes: | A. Almamy, C. Schwerk, H. Schroten, H. Ishikawa, A. R. Asif, B. Reuss |
| Summary: | Early maternal infections with Neisseria gonorrhoeae (NG) correlate to an increased lifetime schizophrenia risk for the offspring, which might be due to an immune-mediated mechanism. Here, we investigated the interactions of polyclonal antisera to NG (α-NG) with a first trimester prenatal brain multiprotein array, revealing among others the SNARE-complex protein Snap23 as a target antigen for α-NG. This interaction was confirmed by Western blot analysis with a recombinant Snap23 protein, whereas the closely related Snap25 failed to interact with α-NG. Furthermore, a polyclonal antiserum to the closely related bacterium Neisseria meningitidis (α-NM) failed to interact with both proteins. Functionally, in SH-SY5Y cells, α-NG pretreatment interfered with both insulin-induced vesicle recycling, as revealed by uptake of the fluorescent endocytosis marker FM1-43, and insulin-dependent membrane translocation of the glucose transporter GluT4. Similar effects could be observed for an antiserum raised directly to Snap23, whereas a serum to Snap25 failed to do so. In conclusion, Snap23 seems to be a possible immune target for anti-gonococcal antibodies, the interactions of which seem at least in vitro to interfere with vesicle-associated exocytosis. Whether these changes contribute to the correlation between maternal gonococcal infections and psychosis in vivo remains still to be clarified. |
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| Item Description: | Gesehen am 30.04.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1559-1166 |
| DOI: | 10.1007/s12031-017-0920-2 |