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Continuous stimulation with differentiation factors is necessary to enhance osteogenic differentiation of human mesenchymal stem cells in-vitro

Bone defect treatment belongs to the most challenging fields in orthopedic surgery and requires the well-coordinated application of mesenchymal stem cells (MSC) and differentiation factors. MSC isolated from reaming material (RMSC) and iliac crest (BMSC) in combination with bone morphogenetic protei...

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Bibliographic Details
Main Authors: Reible, Bruno (Author) , Schmidmaier, Gerhard (Author) , Prokscha, Matthäus (Author) , Moghaddam-Alvandi, Arash (Author) , Westhauser, Fabian (Author)
Format: Article (Journal)
Language:English
Published: 12 December 2017
In: Growth factors
Year: 2017, Volume: 35, Issue: 4/5, Pages: 179-188
ISSN:1029-2292
DOI:10.1080/08977194.2017.1401618
Online Access:Verlag, Volltext: http://dx.doi.org/10.1080/08977194.2017.1401618
Verlag, Volltext: https://doi.org/10.1080/08977194.2017.1401618
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Author Notes:Bruno Reible, Gerhard Schmidmaier, Matthäus Prokscha, Arash Moghaddam and Fabian Westhauser
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Summary:Bone defect treatment belongs to the most challenging fields in orthopedic surgery and requires the well-coordinated application of mesenchymal stem cells (MSC) and differentiation factors. MSC isolated from reaming material (RMSC) and iliac crest (BMSC) in combination with bone morphogenetic protein-7 (BMP-7) and insulin-like growth factor-1 (IGF-1) have been used. The short half-life of both factors limit their applications: a burst release of the factor can probably not induce sustainable differentiation. We stimulated MSC in osteogenic differentiation medium with three different concentrations of BMP-7 or IGF-1: Group A was stimulated continuously, group B for 24 h and group C remained without any stimulation. Osteogenic differentiation was measured after seven and 14 days by alizarin red staining and alkaline phosphatase (ALP) activity. Continuous stimulation led to higher levels of osteogenic differentiation than short-term stimulation. This could lead to a reconsideration of established application forms for differentiation factors, aiming to provide a more sustained release.
Item Description:Gesehen am 02.05.2018
Physical Description:Online Resource
ISSN:1029-2292
DOI:10.1080/08977194.2017.1401618

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