Functional analysis of recombinant channels in host cells using a fast agonist application system
A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004)...
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| Main Authors: | , |
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| Format: | Article (Journal) Chapter/Article |
| Language: | English |
| Published: |
2017
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| In: |
NMDA Receptors
Year: 2017, Pages: 163-169 |
| DOI: | 10.1007/978-1-4939-7321-7_8 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/978-1-4939-7321-7_8
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| Author Notes: | Andrei Rozov, David Jappy |
| Summary: | A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261-287, 1992; Jonas and Sakmann, J Physiol 455:143-171, 1992). |
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| Item Description: | Gesehen am 02.05.2018 |
| Physical Description: | Online Resource |
| ISBN: | 9781493973217 |
| DOI: | 10.1007/978-1-4939-7321-7_8 |