Evaluation of the dynamicity of mitotic exit network and spindle position checkpoint components on spindle pole bodies by Fluorescence Recovery After Photobleaching (FRAP)
Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study in vivo binding and diffusion dynamics of fluorescently labeled proteins. In this chapter, we describe how to determine spindle pole body (SPB) binding dynamics of mitotic exit network (MEN) and spindle position check...
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| Main Authors: | , |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
[2017]
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| In: |
The Mitotic Exit Network
Year: 2017, Pages: 167-182 |
| Online Access: | Verlag, Volltext: https://link.springer.com/protocol/10.1007/978-1-4939-6502-1_13
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| Author Notes: | Ayse Koca Caydasi, Gislene Pereira |
| Summary: | Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study in vivo binding and diffusion dynamics of fluorescently labeled proteins. In this chapter, we describe how to determine spindle pole body (SPB) binding dynamics of mitotic exit network (MEN) and spindle position checkpoint (SPOC) proteins using FRAP microscopy. Procedures presented here include the growth of the yeast cultures, sample preparation, image acquisition and analysis. |
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| Item Description: | Gesehen am 21.12.2020 |
| Physical Description: | Online Resource |
| ISBN: | 9781493965021 |