Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations
Objectives: To characterize the localization profiles of seven adhesion/growth-regulatory galectins in the normal human eye using immunodetection of endogenous galectins and binding of labeled galectins to sections, useful as a basis to detect disease-associated alterations.MethodsNon-cross-reactive...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
25 April 2012
|
| In: |
Graefe's archive for clinical and experimental ophthalmology
Year: 2012, Volume: 250, Issue: 8, Pages: 1169-1180 |
| ISSN: | 1435-702X |
| DOI: | 10.1007/s00417-012-2021-9 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s00417-012-2021-9 Verlag, Volltext: https://link.springer.com/article/10.1007/s00417-012-2021-9 
|
| Author Notes: | Ursula Schlötzer-Schrehardt, Sabine André, Christina Janko, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius, Martin Herrmann |
| Summary: | Objectives: To characterize the localization profiles of seven adhesion/growth-regulatory galectins in the normal human eye using immunodetection of endogenous galectins and binding of labeled galectins to sections, useful as a basis to detect disease-associated alterations.MethodsNon-cross-reactive anti-galectin antibodies and biotinylated galectins were tested on acetone-fixed cryosections of normal human donor eyes. Controls included omission of first-step reagent, testing of an antibody against a galectin specific for rat (galectin-5), and blocking of galectin binding with lactose.ResultsGalectin presence was not restricted to one or few members of this family. Signal occurrence can even include all tested or most proteins (conjunctival or corneal epithelium), whereas choroid positivity is fully accounted for by galectin-9. Regional specificity and characteristic profiles for each protein, immuno- and galectin histochemically, were determined. Differences in tissue reactivity among the galectins were detected.ConclusionsThat the galectins have characteristic localization/reactivity profiles supports the concept of a network with potential for non-overlapping functions. The reported data thus prompt to proceed to the respective analysis of specimens from ocular diseases. |
|---|---|
| Item Description: | Gesehen am 14.05.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1435-702X |
| DOI: | 10.1007/s00417-012-2021-9 |