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Clustering of subgingival microbiota reveals microbial disease ecotypes associated with clinical stages of periodontitis in a cross-sectional study

Periodontitis is characterized by chronic inflammation associated with alteration of the oral microbiota. In contrast to previous microbiome studies focusing a priori on comparison between extreme phenotypes, our study analyzed a random sample of 85 people. The aim of this study was to link microbia...

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Main Authors: Boutin, Sébastien (Author) , Hagenfeld, Daniel (Author) , Zimmermann, Heiko (Author) , Sayed, Nihad el (Author) , Becher, Heiko (Author) , Kim, Ti-Sun (Author) , Dalpke, Alexander (Author)
Format: Article (Journal)
Language:English
Published: 01 March 2017
In: Frontiers in microbiology
Year: 2017, Volume: 8
ISSN:1664-302X
DOI:10.3389/fmicb.2017.00340
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.3389/fmicb.2017.00340
Verlag, kostenfrei, Volltext: https://www.frontiersin.org/articles/10.3389/fmicb.2017.00340/full
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Author Notes:Sébastien Boutin, Daniel Hagenfeld, Heiko Zimmermann, Nihad El Sayed, Tanja Höpker, Halina K. Greiser, Heiko Becher, Ti-Sun Kim, Alexander H. Dalpke
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Summary:Periodontitis is characterized by chronic inflammation associated with alteration of the oral microbiota. In contrast to previous microbiome studies focusing a priori on comparison between extreme phenotypes, our study analyzed a random sample of 85 people. The aim of this study was to link microbial differences to disease’s prevalence and severity. Using next generation sequencing of 16S rRNA amplicons and cluster analysis, we observed that the population can be divided into two major ecotypes: One mainly contained periodontal healthy/mild periodontitis individuals whereas the second ecotype showed a heterogeneous microbial distribution and clustered into three distinct sub-ecotypes. Those sub-ecotypes differed with respect to the frequency of diseased patients and displayed a gradual change in distinct subgingival microbiota that goes along with clinical disease symptoms. In ecotype 2, the subgroup with no clinical signs of disease was linked to an increase of F. nucleatum vincentii but also several other species, while only in “end-stage” dysbiosis classical red complex bacteria gained overweight. Therefore, the microbial disease ecotypes observed in our population can lead to an establishment of an early microbial risk profile for clinically healthy patients.
Item Description:Gesehen am 15.05.2018
Physical Description:Online Resource
ISSN:1664-302X
DOI:10.3389/fmicb.2017.00340

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