Cryo-Electron Microscopy Modeling by the Molecular Dynamics Flexible Fitting Method
The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
28 February 2012
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| In: |
Biopolymers
Year: 2012, Volume: 97, Issue: 9, Pages: 678-686 |
| ISSN: | 1097-0282 |
| DOI: | 10.1002/bip.22042 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1002/bip.22042 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/bip.22042 
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| Author Notes: | Kwok-Yan Chan, Leonardo G. Trabuco, Eduard Schreiner, Klaus Schulten |
| Summary: | The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge 2010 is the first community effort to bring together developers of hybrid methods as well as cryo-EM experimentalists. Participating in the challenge, the molecular dynamics flexible fitting (MDFF) method was applied to a number of cryo-EM density maps. The results are described here with special emphasis on the use of symmetry-based restraints to improve the quality of atomic models derived from density maps of symmetric complexes; on a comparison of the stereochemical quality of atomic models resulting from different hybrid methods; and on application of MDFF to electron crystallography data. © 2012 Wiley Periodicals, Inc. Biopolymers 97: 678-686, 2012. |
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| Item Description: | Gesehen am 15.05.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1097-0282 |
| DOI: | 10.1002/bip.22042 |