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Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes

Disturbances in vitamin A metabolism are an important attribute of some cancer cells. Most evidence point that these disturbances lead to decreasing of the retinoic acid concentration in tumor cells. Up to now, in benign and malignant skin cells the features of vitamin A metabolism with its particip...

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Bibliographic Details
Main Authors: Amann, Philipp M. (Author) , Freudenberger, Muriel (Author) , Holland-Cunz, Stefan (Author) , Eichmüller, Stefan B. (Author) , Bazhin, Alexandr V. (Author)
Format: Article (Journal)
Language:English
Published: 2012
In: Journal of cellular biochemistry
Year: 2012, Volume: 113, Issue: 3, Pages: 792-799
ISSN:1097-4644
DOI:10.1002/jcb.23406
Online Access:Verlag, Volltext: http://dx.doi.org/10.1002/jcb.23406
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Author Notes:Philipp M. Amann, Claudia Hofmann, Muriel Freudenberger, Stefan Holland-Cunz, Stefan B. Eichmüller, and Alexandr V. Bazhin
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Summary:Disturbances in vitamin A metabolism are an important attribute of some cancer cells. Most evidence point that these disturbances lead to decreasing of the retinoic acid concentration in tumor cells. Up to now, in benign and malignant skin cells the features of vitamin A metabolism with its participating enzymes are not entirely understood. Alcohol and aldehyde dehydrogenases (ALDH) are involved in the retinol metabolism, oxidizing retinol, and retinal in retinoic acid or reducing retinal in retinol. In this work we investigated the expression and enzymatic activity of alcohol and ALDH in melanoma cells compared to their benign counterparts. We demonstrated that melanoma cell lines and melanocytes despite similar pattern of the enzyme expression, show different general ALDH activity. Retinal, the substrate of ALDH, could stimulate the ALDH activity through up-regulation of retinaldehyde dehydrogenase 1 and aldehyde dehydrogenase 6. Furthermore, we found that retinoids regulate alcohol dehydrogenase activity, probably via effects on alcohol dehydrogenase expression at the post-transcriptional level. We suggest that melanoma cells in contrast to melanocytes should favor the retinal reduction over its oxidation. The decreasing cellular amount of the precursor molecules of retinoic acid could result in a changed gene regulation in melanoma cells.
Item Description:First published: 20 October 2011
Gesehen am 17.05.2018
Physical Description:Online Resource
ISSN:1097-4644
DOI:10.1002/jcb.23406

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