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Evidence for the subsynaptic zone as a preferential site for CHRN recycling at neuromuscular junctions

Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in viv...

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Bibliographic Details
Main Authors: Wild, Franziska (Author) , Khan, Muzamil Majid (Author) , Rudolf, Rüdiger (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: Small GTPases
Year: 2017, Volume: 10, Issue: 5, Pages: 1-8
ISSN:2154-1256
DOI:10.1080/21541248.2017.1324939
Online Access:Verlag, Volltext: http://dx.doi.org/10.1080/21541248.2017.1324939
Verlag, Volltext: https://doi.org/10.1080/21541248.2017.1324939
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Author Notes:Franziska Wild, Muzamil Majid Khan, Rüdiger Rudolf
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Summary:Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in vivo-imaging of endocytosed CHRN and RAB-GFP fusion proteins, we deliver evidence for differential effects of RAB5-GFP, RAB4-GFP, and RAB11-GFP on CHRN endocytosis. Furthermore, while newly endocytosed CHRN colocalized with RAB5-GFP over large stretches of muscle fibers, RAB4-GFP and RAB11-GFP colocalized with endocytosed CHRN almost exclusively at neuromuscular junctions. In agreement with previous findings, this data suggests the existence of a specialized subsynaptic zone that is particularly relevant for CHRN recycling.
Item Description:Published online: 08 Jun 2017
Gesehen am 04.06.2018
Physical Description:Online Resource
ISSN:2154-1256
DOI:10.1080/21541248.2017.1324939

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