Evidence for the subsynaptic zone as a preferential site for CHRN recycling at neuromuscular junctions
Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in viv...
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| Autori principali: | , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
2019
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| In: |
Small GTPases
Year: 2017, Volume: 10, Fascicolo: 5, Pages: 1-8 |
| ISSN: | 2154-1256 |
| DOI: | 10.1080/21541248.2017.1324939 |
| Accesso online: | Verlag, Volltext: http://dx.doi.org/10.1080/21541248.2017.1324939 Verlag, Volltext: https://doi.org/10.1080/21541248.2017.1324939 
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| Note sull'autore: | Franziska Wild, Muzamil Majid Khan, Rüdiger Rudolf |
| Riassunto: | Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in vivo-imaging of endocytosed CHRN and RAB-GFP fusion proteins, we deliver evidence for differential effects of RAB5-GFP, RAB4-GFP, and RAB11-GFP on CHRN endocytosis. Furthermore, while newly endocytosed CHRN colocalized with RAB5-GFP over large stretches of muscle fibers, RAB4-GFP and RAB11-GFP colocalized with endocytosed CHRN almost exclusively at neuromuscular junctions. In agreement with previous findings, this data suggests the existence of a specialized subsynaptic zone that is particularly relevant for CHRN recycling. |
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| Descrizione del documento: | Published online: 08 Jun 2017 Gesehen am 04.06.2018 |
| Descrizione fisica: | Online Resource |
| ISSN: | 2154-1256 |
| DOI: | 10.1080/21541248.2017.1324939 |