Biological reproducibility of circulating P-Selectin, Thrombopoietin, GPIIb/IIIa and Thrombomodulin over one year
Background: Enhanced platelet activation has been implicated in several pathophysiological processes. Here, we evaluated the biological reproducibility of circulating P-Selectin, Thrombomodulin (TM), Thrombopoietin (TPO), and Glycoprotein IIb/IIIa (GPIIb/IIIa) to assess whether these analytes can be...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2017
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| In: |
Clinical biochemistry
Year: 2017, Volume: 50, Issue: 16, Pages: 942-946 |
| ISSN: | 1873-2933 |
| DOI: | 10.1016/j.clinbiochem.2017.05.017 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.clinbiochem.2017.05.017 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0009912017302515 
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| Author Notes: | Mirja E. Graf, Disorn Sookthai, Theron Johnson, Ruth Schübel, Verena Katzke, Peter Bugert, Michael Hoffmeister, Rudolf Kaaks, Tilman Kühn |
| Summary: | Background: Enhanced platelet activation has been implicated in several pathophysiological processes. Here, we evaluated the biological reproducibility of circulating P-Selectin, Thrombomodulin (TM), Thrombopoietin (TPO), and Glycoprotein IIb/IIIa (GPIIb/IIIa) to assess whether these analytes can be used as reliable biomarkers of platelet activation in epidemiological studies. Methods: We measured circulating P-Selectin, TM, TPO and GPIIb/IIIa by immunoassays in two blood samples of 78 participants of the EPIC Heidelberg study (47-80years, 50% female) that were collected one year apart. Biological reproducibility of biomarker levels over time and associations with routine biochemistry parameters were assessed by Spearman's correlation coefficients. Results: Statistical analyses revealed good reproducibility over one year for two of the analyzed markers, with Spearman coefficients of ρ=0.80 (P-Selectin) and ρ=0.73 (TPO) and reasonable reproducibility for TM (ρ=0.63) and GPIIb/IIIa (ρ=0.51). Levels of P-Selectin, TM, TPO and GPIIb/IIIa were not significantly associated with routine biochemistry parameters, such as glucose, HbA1c, LDL, HDL, Triglycerides and CRP. Conclusions: Our findings suggest that a single assessment of P-Selectin, TM, TPO and GPIIb/IIIa at baseline in prospective epidemiological studies is appropriate to investigate associations between platelet activation and risks of chronic diseases. |
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| Item Description: | Gesehen am 19.06.2018 Available online 29 May 2017 |
| Physical Description: | Online Resource |
| ISSN: | 1873-2933 |
| DOI: | 10.1016/j.clinbiochem.2017.05.017 |