In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice
SUMOylation, an essential posttranslational protein modification, is involved in many eukaryotic cellular signaling pathways. The identification of SUMOylated proteins is difficult, because SUMOylation sites in proteins are hard to predict, SUMOylated protein states are transient in vivo and labile...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
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| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 2012, Jahrgang: 109, Heft: 51, Pages: 21122-21127 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.1215366110 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1073/pnas.1215366110 Verlag, kostenfrei, Volltext: http://www.pnas.org/content/109/51/21122 
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| Verfasserangaben: | Marilyn Tirard, He-Hsuan Hsiao, Miroslav Nikolov, Henning Urlaub, Frauke Melchior, and Nils Brose |
| Zusammenfassung: | SUMOylation, an essential posttranslational protein modification, is involved in many eukaryotic cellular signaling pathways. The identification of SUMOylated proteins is difficult, because SUMOylation sites in proteins are hard to predict, SUMOylated protein states are transient in vivo and labile in vitro, only a small substrate fraction is SUMOylated in vivo, and identification tools for natively SUMOylated proteins are rare. To solve these problems, we generated knock-in mice expressing His6-HA-SUMO1. By anti-HA immunostaining, we show that SUMO1 conjugates in neurons are only detectable in nuclei and annulate lamellae. By anti-HA affinity purification, we identified several hundred candidate SUMO1 substrates, of which we validated Smchd1, Ctip2, TIF1γ, and Zbtb20 as novel substrates. The knock-in mouse represents an excellent mammalian model for studies on SUMO1 localization and screens for SUMO1 conjugates in vivo. |
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| Beschreibung: | Published first December 3, 2012 Gesehen am 21.06.2018 Im Titel ist bei "His6-HA-SUMO1" die 6 tiefgestellt |
| Beschreibung: | Online Resource |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.1215366110 |