Cover Image

Carbon ion irradiation inhibits glioma cell migration through downregulation of integrin expression

Purpose To investigate the effect of carbon ion irradiation on glioma cell migration. Methods and Materials U87 and Ln229 glioma cells were irradiated with photons and carbon ions. Migration was analyzed 24 h after irradiation. Fluorescence-activated cell sorting analysis was performed in order to q...

Full description

Saved in:
Bibliographic Details
Main Authors: Rieken, Stefan (Author) , Habermehl, Daniel (Author) , Würth, Lena (Author) , Brons, Stephan (Author) , Mohr, Angela (Author) , Lindel, Katja (Author) , Weber, Klaus-Josef (Author) , Haberer, Thomas (Author) , Debus, Jürgen (Author) , Combs, Stephanie (Author)
Format: Article (Journal)
Language:English
Published: 2012
In: International journal of radiation oncology, biology, physics
Year: 2012, Volume: 83, Issue: 1, Pages: 394-399
ISSN:1879-355X
DOI:10.1016/j.ijrobp.2011.06.2004
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.ijrobp.2011.06.2004
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0360301611030045
Get full text
Author Notes:Stefan Rieken, Daniel Habermehl, Lena Wuerth, Stephan Brons, Angela Mohr, Katja Lindel, Klaus Weber, Thomas Haberer, Jürgen Debus, and Stephanie E. Combs
Description
Summary:Purpose To investigate the effect of carbon ion irradiation on glioma cell migration. Methods and Materials U87 and Ln229 glioma cells were irradiated with photons and carbon ions. Migration was analyzed 24 h after irradiation. Fluorescence-activated cell sorting analysis was performed in order to quantify surface expression of integrins. Results Single photon doses of 2 Gy and 10 Gy enhanced ανβ3 and ανβ5 integrin expression and caused tumor cell hypermigration on both vitronectin (Vn) and fibronectin (Fn). Compared to integrin expression in unirradiated cells, carbon ion irradiation caused decreased integrin expression and inhibited cell migration on both Vn and Fn. Conclusion Photon radiotherapy (RT) enhances the risk of tumor cell migration and subsequently promotes locoregional spread via photon induction of integrin expression. In contrast to photon RT, carbon ion RT causes decreased integrin expression and suppresses glioma cell migration on both Vn and Fn, thus promising improved local control.
Item Description:Available online 4 November 2011
Gesehen am 22.06.2018
Physical Description:Online Resource
ISSN:1879-355X
DOI:10.1016/j.ijrobp.2011.06.2004

Similar Items